Sex differences in striatal dopaminergic function within heterozygous mutant dopamine transporter knock-out mice.

Title

Sex differences in striatal dopaminergic function within heterozygous mutant dopamine transporter knock-out mice.

Creator

Ji Jing; Dluzen Dean E

Publisher

Journal of neural transmission (Vienna, Austria : 1996)

Date

2008
2008-06

Description

The issue of whether a deletion of the dopamine transporter (DAT) allele (+/- DAT) would differentially alter striatal dopamine (DA) and dihydroxyphenylacetic acid (DOPAC) concentrations and DA release upon potassium and methamphetamine (MA) stimulation between male and female mice was examined. Striatal DA and DOPAC concentrations of female +/- DAT mice were significantly decreased as compared with wild type (+/+) controls and male +/- DAT mice. No such changes were obtained from the olfactory tubercle suggesting that these effects might be specific for the striatum. Potassium-stimulated DA was increased in male and female +/- DAT mice and maximally stimulated DA was obtained from +/- DAT females, although these mice showed the lowest DA concentrations. MA-evoked DA was increased in male and female +/- mice. While MA-evoked DA was significantly increased in +/+ males versus +/+ females, the +/- females showed the highest DA responses, thereby showing a reversal in the results seen in wild-type conditions. These findings indicate: (1) that a deficiency in the DAT interacts with the sex of the subject, (2)+/- DAT females show more extreme changes in dopaminergic responses, and (3) the importance for considering such variables such as sex when examining differences among knock-out conditions.

Subject

*Sex Characteristics; 3; 4-Dihydroxyphenylacetic Acid/metabolism; Animals; Brain/drug effects/*metabolism; Corpus Striatum/drug effects/*metabolism; Dopamine Plasma Membrane Transport Proteins/*genetics; Dopamine Uptake Inhibitors/pharmacology; Dopamine/*metabolism; Down-Regulation/drug effects/genetics; Female; Gene Expression Regulation/drug effects/genetics; Heterozygote; Knockout; Male; Methamphetamine/pharmacology; Mice; Mutation/*genetics; Neural Pathways/drug effects/metabolism; Potassium Chloride/metabolism/pharmacology; Substantia Nigra/drug effects/metabolism; Synaptic Transmission/drug effects/genetics; Up-Regulation/drug effects/genetics

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

809–817

Issue

6

Volume

115

Citation

Ji Jing; Dluzen Dean E, “Sex differences in striatal dopaminergic function within heterozygous mutant dopamine transporter knock-out mice.,” NEOMED Bibliography Database, accessed April 22, 2021, https://neomed.omeka.net/items/show/3205.

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