Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.

Title

Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.

Creator

Sutariya Vijaykumar; Wehrung Daniel; Geldenhuys Werner J

Publisher

Journal of chromatographic science

Date

2012
2012-03

Description

The objective of this study was the development, optimization, and validation of a novel reverse-phase high-pressure liquid chromatography (RP-HPLC) method for the quantification of reduced glutathione in pharmaceutical formulations utilizing simple UV detection. The separation utilized a C18 column at room temperature and UV absorption was measured at 215 nm. The mobile phase was an isocratic flow of a 50/50 (v/v) mixture of water (pH 7.0) and acetonitrile flowing at 1.0 mL/min. Validation of the method assessed the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. Analysis of the system suitability showed acceptable levels of suitability in all categories. Likewise, the method displayed an acceptable degree of linearity (r(2) = 0.9994) over a concentration range of 2.5-60 microg/mL. The detection limit and quantification limit were 0.6 and 1.8 microg/mL respectively. The percent recovery of the method was 98.80-100.79%. Following validation the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a conjugate and a nanoparticle. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.

Subject

Chromatography; Glutathione/*analysis; High Pressure Liquid/*methods; Limit of Detection; Linear Models; Nanoparticles/chemistry; Pharmaceutical Preparations/chemistry; Reproducibility of Results; Reverse-Phase/*methods; Spectrophotometry; Ultraviolet

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

271–276

Issue

3

Volume

50

Citation

Sutariya Vijaykumar; Wehrung Daniel; Geldenhuys Werner J, “Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.,” NEOMED Bibliography Database, accessed June 19, 2021, https://neomed.omeka.net/items/show/4171.

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