Surface Enhanced Raman Spectroscopy (SERS) for the Multiplex Detection of Braf, Kras, and Pik3ca Mutations in Plasma of Colorectal Cancer Patients.

Surface Enhanced Raman Spectroscopy (SERS) for the Multiplex Detection of Braf, Kras, and Pik3ca Mutations in Plasma of Colorectal Cancer Patients.

Li Xiaozhou; Yang Tianyue; Li Caesar Siqi; Song Youtao; Lou Hong; Guan Dagang; Jin Lili

Theranostics

2018

1905-07

In this paper, we discuss the use of a procedure based on polymerase chain reaction (PCR) and surface enhanced Raman spectroscopy (SERS) (PCR-SERS) to detect DNA mutations. Methods: This method was implemented by first amplifying

Adult; Female; Humans; Male; Middle Aged; Aged; *colorectal cancer; *gene; *mutation; *Mutation; *PCR; *surface enhanced Raman scattering; Class I Phosphatidylinositol 3-Kinases/blood/*genetics; Cluster Analysis; Colorectal Neoplasms/blood/*diagnosis/genetics/pathology; DNA Primers/chemistry; Exons; Multiplex Polymerase Chain Reaction/methods/standards; Neoplasm Staging; Proto-Oncogene Proteins B-raf/blood/*genetics; Proto-Oncogene Proteins p21(ras)/blood/*genetics; Biomarkers; Spectrum Analysis; Raman/methods/*standards; Tumor/blood/*genetics

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1678–1689

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