Castration differentially alters [3H]nisoxetine binding to norepinephrine uptake sites in olfactory bulb and frontal cortex of male rats.

Castration differentially alters [3H]nisoxetine binding to norepinephrine uptake sites in olfactory bulb and frontal cortex of male rats.

Shang Y; Boja J W; Dluzen D E

Synapse (New York, N.Y.)

1999

1999-03

In the present study, [3H]nisoxetine binding to norepinephrine (NE) uptake sites and [3H]norepinephrine uptake were investigated within olfactory bulb (OB) and frontal cortex homogenates from intact and castrated male rats. Statistically significant reductions in the number of [3H]nisoxetine binding sites (Bmax) were found in OB from the castrates, while significantly increased Bmax values were obtained in the frontal cortex. Castration also significantly altered the affinity (Kd) of [3H]nisoxetine binding in the frontal cortex, but not in the OB. Assessment of [3H]norepinephrine uptake showed that in neither brain regions were there any statistically significant differences in Km nor Vmax between the castrated and intact male rats, indicating that the basal uptake process is not changed following castration in either of these brain areas. These results demonstrate the differential effects of castration upon [3H]nisoxetine binding sites between the OB and frontal cortex. Such findings provide new evidence for one of the mechanisms by which androgens may modulate central noradrenergic activity.

*Orchiectomy; Adrenergic/drug effects/*metabolism; Animals; Fluoxetine/*analogs & derivatives/metabolism; Frontal Lobe/drug effects/*metabolism; Kinetics; Male; Norepinephrine/metabolism; Olfactory Bulb/drug effects/*metabolism; Rats; Receptors; Sprague-Dawley; Synaptosomes/drug effects/metabolism; Testosterone/pharmacology

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