Fetal lung epithelial ion channels relocate in the cell membrane during late gestation.

Title

Fetal lung epithelial ion channels relocate in the cell membrane during late gestation.

Creator

Beard LaMonta L; Li Tianbo; Hu Yang; Folkesson Hans G

Publisher

Anatomical record (Hoboken, N.J. : 2007)

Date

2011
2011-09

Description

Near the end of gestation, the direction of ion and fluid flow across the alveolar epithelium rapidly changes from secretion to absorption. Thus, the relative cell membrane location of epithelial Na channels (ENaCs) and cystic fibrosis transmembrane regulator (CFTR) Cl channels during late fetal lung development and after maternal interleukin-1beta (IL-1beta) pretreatment was the focus of our study. Western blot analysis after sucrose gradient separation of caveolin-1-(Cav-1)-rich membrane regions (CRR) and Cav-1-poor membrane (non-CRR) revealed primary CRR ENaC localization at gestation day (GD) 61 in guinea pigs. Correlating with the natural induction of distal lung fluid absorption, ENaC appeared in the non-CRR cell membrane regions at GD68. Conversely, CFTR was present in the non-CRR cell membrane regions at GD61 and in the CRRs at GD68.

Subject

Animals; Blotting; Caveolin 1/*metabolism; Cell Membrane/*metabolism; Cl transport; Cystic Fibrosis Transmembrane Conductance Regulator/*metabolism; distal lung fluid absorption; Epithelial Sodium Channels/*metabolism; Epithelium/embryology/*metabolism; Female; fetal lung development; Fetus/*metabolism; Gestational Age; Guinea Pigs; IL-1beta; Immunoprecipitation; Interleukin-1beta/metabolism; Ion Channels/metabolism; Lung/embryology/*metabolism; Na transport; Western

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1461–1471

Issue

9

Volume

294

Citation

Beard LaMonta L; Li Tianbo; Hu Yang; Folkesson Hans G, “Fetal lung epithelial ion channels relocate in the cell membrane during late gestation.,” NEOMED Bibliography Database, accessed April 20, 2021, https://neomed.omeka.net/items/show/2966.

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