Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.

Title

Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.

Creator

Giordano S J; Steggles A W

Publisher

Biochimica et biophysica acta

Date

1993
1993-02

Description

Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed. Both hybridized strongly to a radiolabelled liver cytochrome b5 probe. Sequencing confirmed that the two types of cDNA product encoded the membrane-bound and the soluble forms of b5. Messenger RNA corresponding to the soluble cytochrome was detected in the lung, gallbladder and the adrenal gland, as well as in reticulocytes and bone marrow. This was an unexpected finding since the protein has been isolated only from erythrocytes. In contrast, membrane-bound cytochrome b5 mRNA was detected in all tissues tested, suggesting that the corresponding protein is ubiquitous in tissue distribution.

Subject

Agar Gel; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cell Membrane/metabolism; Cytochromes b5/*genetics; Cytosol/metabolism; DNA/genetics/isolation & purification; Electrophoresis; Exons; Leukocytes/metabolism; Liver/*metabolism; Messenger/genetics/isolation & purification/*metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction/methods; Rabbits; Reticulocytes/metabolism; RNA; Southern

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

95–100

Issue

1

Volume

1172

Citation

Giordano S J; Steggles A W, “Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.,” NEOMED Bibliography Database, accessed September 17, 2021, https://neomed.omeka.net/items/show/3348.

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