Browse Items · NEOMED Bibliography Database

The complete nucleotide sequence of human liver cytochrome b5 mRNA.

We have isolated and sequenced a cDNA clone corresponding to human liver cytochrome b5 mRNA. The 760 base pair (bp) sequence contains the complete coding and 3' non-translated regions plus 52 bp of 5' non-translated sequence. The derived amino acid…

Tags: 1988, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, DNA/genetics, Humans, Liver/*metabolism, Messenger/*genetics, Molecular, Molecular Sequence Data, RNA, Steggles A W, Yoo M

The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.

We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A)…

Tags: 1989, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, DNA/genetics, Humans, Liver/*physiology, Molecular, Molecular Sequence Data, Post-Transcriptional, Pseudogenes, RNA Processing, Steggles A W, Yoo M

Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.

Oxysterol 7 alpha-hydroxylase catalyzes hydroxylation of oxysterols and neurosterols and plays a role in the alternative bile acid synthesis pathway. This gene is widely expressed in many organs and peripheral tissues and may protect tissues from the…

Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.

Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA…

Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.

A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol

Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.

Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of…

The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.

We have recently cloned a full-length cDNA encoding the rat hepatic cholesterol 7 alpha-hydroxylase cytochrome P450 (P450c7) (Li, Y. C., Wang, D. P., and Chiang, J. Y. L. (1990) J. Biol. Chem. 265, 12012-12019), which catalyzes the rate-limiting…

Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.

We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two…

Polymorphisms of human cholesterol 7 alpha-hydroxylase.

Human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs were isolated from a human liver cDNA library. A full-length cDNA has 2901 nucleotides which encode a typical P450 polypeptide of 504 amino acid residues. Two different sequences of codon 100,…

Tags: 1992, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, Cloning, Department of Integrative Medical Sciences, DNA/genetics, Genetic, Humans, Karam W G, Molecular, Molecular Sequence Data, NEOMED College of Medicine, Oligodeoxyribonucleotides/chemistry, Polymerase Chain Reaction, Polymorphism, Restriction Mapping

Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Cholesterol 7 alpha-hydroxylase (P450c7) is the first and rate-limiting enzyme in bile acid biosynthesis and is the product of a cytochrome P450 gene, CYP7. We have previously reported the cloning of a full-length human cholesterol 7…

Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.

RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products…

Tags: 1992, Amino Acid Sequence, Animals, Base Sequence, Cytochromes b5/chemistry/*genetics, Giordano S J, Leukocytes/*chemistry, Liver/chemistry, Messenger/*chemistry, Molecular Sequence Data, Nucleic Acid, Nucleic Acid Hybridization, Polymerase Chain Reaction, Rabbits, Reticulocytes/*chemistry, RNA, SAAS bulletin, biochemistry and biotechnology, Sequence Homology, Steggles A W

The nucleotide sequence of rabbit liver cytochrome b5 mRNA.

Using a synthetic 26 base pair (bp) oligonucleotide, we have screened a rabbit liver cDNA library in lambda gt11 and isolated a 1000 bp DNA clone corresponding to cytochrome b5 mRNA. The clone contains the complete coding region and long 5' and 3'…

Tags: 1988, Amino Acid Sequence, Animals, Base Sequence, Chromosome Deletion, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, Dariush N, Fisher C W, Genes, Liver/*metabolism, Messenger/*genetics, Molecular, Molecular Sequence Data, Protein sequences & data analysis, Rabbits, RNA, Steggles A W

Isolation and characterization of cDNA clones for human, bovine and rabbit liver cytochrome b5 mRNA's.

The complete nucleotide sequence of bovine liver cytochrome b5 mRNA.

Tags: 1989, Amino Acid Sequence, Animals, Base Sequence, Cattle, Cristiano R J, Cytochrome b Group/*genetics/isolation & purification, Cytochromes b5, Liver/*enzymology, Messenger/*isolation & purification, Molecular Sequence Data, Nucleic acids research, RNA, Steggles A W

Transcriptional activation of the cholesterol 7alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors.

The gene encoding cholesterol 7alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the…

Tags: *Receptors, *Transcriptional Activation, 1998, Animals, Base Sequence, Bile Acids and Salts/biosynthesis, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, COUP Transcription Factor II, COUP Transcription Factors, Crestani M, Cultured, Cytoplasmic and Nuclear/*physiology, Department of Integrative Medical Sciences, DNA-Binding Proteins/metabolism, DNA/metabolism, Galli G, Genes, Genetic, Hepatocyte Nuclear Factor 4, Hormones/*physiology, Journal of lipid research, Luciferases/genetics, Molecular Sequence Data, Mutagenesis, NEOMED College of Medicine, Nucleic Acid, Oligonucleotide Probes/metabolism, Phosphoproteins/metabolism, Promoter Regions, Rats, Receptors, Repetitive Sequences, Reporter, Retinoic Acid/metabolism, Retinoid X Receptors, Sadeghpour A, Site-Directed, Steroid, Stroup D, Transcription Factors/metabolism, Tumor Cells

Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).

The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion…

Identification and characterization of a putative bile acid-responsive element in cholesterol 7 alpha-hydroxylase gene promoter.

Nucleotide sequences of a 7997-base pair SacI fragment spanning 3643 base pairs of the upstream promoter region to exon 4 of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) have been determined. DNase I footprinting and electrophoretic mobility…

Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.

A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon…

Tags: *Promoter Regions, 1992, Amino Acid Sequence, Animals, Base Sequence, Biochimica et biophysica acta, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, Cloning, Department of Integrative Medical Sciences, DNA, Genetic, Molecular, Molecular Sequence Data, NEOMED College of Medicine, Rats, Restriction Mapping, Wang D P, Yang T P

Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4alpha (HNF4alpha).

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyses sterol side-chain oxidation of bile acid synthesis from cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27A1 gene…

Tags: *DNA-Binding Proteins, 2003, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Bile Acids and Salts/*pharmacology, Binding Sites/genetics, Cell Line, Chen Wenling, Chenodeoxycholic Acid/pharmacology, Chiang John Y L, Cholestanetriol 26-Monooxygenase, Cloning, Cultured, Cytoplasmic and Nuclear/genetics/metabolism, Department of Integrative Medical Sciences, DNA, DNA/chemistry/genetics, Dose-Response Relationship, Drug, gene, Gene Expression Regulation/drug effects, Genetic/*genetics, Hepatocyte Nuclear Factor 4, Humans, Luciferases/genetics/metabolism, Molecular, Molecular Sequence Data, Mutagenesis, Mutation, NEOMED College of Medicine, Phosphoproteins/genetics/*metabolism, Promoter Regions, Receptors, Recombinant Fusion Proteins/genetics/metabolism, Response Elements/genetics, Sequence Analysis, Site-Directed, Steroid Hydroxylases/*genetics, Transcription Factors/genetics/*metabolism, Transfection, Tumor Cells

Intrastrain variants of herpes simplex virus type 1 isolated from a neonate with fatal disseminated infection differ in the ICP34.5 gene, glycoprotein processing, and neuroinvasiveness.

Two intrastrain variants of herpes simplex virus type 1 (HSV-1) were isolated from a newborn with fatal disseminated infection. A small-plaque-producing variant (SP7) was the predominant virus (\textgreater99%) in the brain, and a…

PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine.

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and

Tags: *Lipid Metabolism, 2007, ATP Binding Cassette Transporter, ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters/genetics, Base Sequence, Cell Line, Chen Wenling, Chiang John Y L, Cholestanetriol 26-Monooxygenase/*metabolism, Cholesterol, Cholesterol/*metabolism, Department of Integrative Medical Sciences, Fluorinated, Genes, Genetic/drug effects, Genetic/genetics, HDL/metabolism, Hepatocytes/drug effects/enzymology/metabolism, Humans, Hydrocarbons, Hydroxycholesterols/metabolism/pharmacology, Intestinal Mucosa/metabolism, Intestines/cytology/drug effects/enzymology, Journal of lipid research, Li Tiangang, Member 1, Messenger/genetics/metabolism, Molecular Sequence Data, NEOMED College of Medicine, Pregnane X Receptor, Promoter Regions, Receptors, Reporter, Response Elements/genetics, Rifampin/pharmacology, RNA, Steroid/*metabolism, Subfamily G, Sulfonamides/pharmacology, Transcription, Up-Regulation/drug effects

IL-1beta-induced cortisol stimulates lung fluid absorption in fetal guinea pigs via SGK-mediated Nedd4-2 inhibition.

We tested the hypothesis that interleukin (IL)-1beta-induced cortisol synthesis stimulates distal lung fluid absorption in fetal guinea pigs via induction of serum- and glucocorticoid-regulated kinase (SGK) and inhibition of neural precursor cell…

Physical map of the tolC-htrP region of the Escherichia coli chromosome.

Tags: *Chromosome Mapping, *Chromosomes, *Escherichia coli Proteins, *Intramolecular Transferases, 1992, Bacterial, Bacterial Outer Membrane Proteins/genetics, Bacterial Proteins/*genetics, Deoxyribonucleases, Depew R E, Escherichia coli/*genetics, Heat-Shock Proteins, Journal of bacteriology, Membrane Transport Proteins, Molecular Sequence Data, Restriction Mapping, Type II Site-Specific/metabolism, Yang T P

Specificity of metabotropic glutamate receptor 2 coupling to G proteins.

Metabotropic glutamate receptor 2 (mGluR2) is a class 3 G protein-coupled receptor and an important mediator of synaptic activity in the central nervous system. Previous work demonstrated that mGluR2 couples to pertussis toxin (PTX)-sensitive G…

Analysis of the thymidine kinase of a herpes simplex virus type 1 isolate that exhibits resistance to (E)-5-(2-bromovinyl)-2'-deoxyuridine.

The mechanism responsible for the decreased sensitivity of a clinical herpes simplex virus type 1 (HSV-1) isolate, HSV-145, to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was examined. Measurements of 50% inhibitory doses of several drugs…

Generation and characterization of ATP analog-specific protein kinase Cdelta.

To better study the role of PKCdelta in normal function and disease, we developed an ATP analog-specific (AS) PKCdelta that is sensitive to specific kinase inhibitors and can be used to identify PKCdelta substrates. AS PKCdelta showed nearly 200…

An N-terminal arginine-rich cluster and a proline-alanine-threonine repeat region determine the cellular localization of the herpes simplex virus type 1 ICP34.5 protein and its ligand, protein phosphatase 1.

The ICP34.5 protein facilitates herpes simplex virus replication by binding and activating protein phosphatase 1 (PP1) by means of a very conserved C-terminal

Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.

Sterol 12alpha-hydroxylase catalyzes the synthesis of cholic acid and controls the ratio of cholic acid over chenodeoxycholic acid in the bile. Transcription of CYP8B1 is inhibited by bile acids, cholesterol, and insulin. To study the mechanism of…

Expression of isotocin-neurophysin mRNA in developing zebrafish.

Neurohypophysial peptides are important regulators of homeostasis, reproduction and behavior. We have sequenced a zebrafish cDNA representing isotocin-neurophysin (IT-NP) mRNA. The developmental expression pattern of zebrafish IT-NP mRNA was…

Tags: 2003, Amino Acid Sequence, Animals, Gene expression patterns : GEP, Glasgow Eric, Messenger/*metabolism, Molecular Sequence Data, Neurophysins/biosynthesis/*genetics, Oxytocin/*analogs & derivatives/biosynthesis/*genetics, Phylogeny, RNA, Unger Jennifer L, Zebrafish/*embryology

Immunization with a LEAPS heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1.

A ligand epitope antigen presentation system (LEAPS) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from beta-2-microglobulin (35-50) elicited protection from…

A L.E.A.P.S. heteroconjugate vaccine containing a T cell epitope from HSV-1 glycoprotein D elicits Th1 responses and protection.

The L.E.A.P.S. heteroconjugate vaccine antigen (JgD), composed of a T cell epitope from glycoprotein D (gD(8-23)) of herpes simplex virus (HSV) linked with a peptide sequence from beta-2-microglobulin (aa38-50), elicited protection against lethal…

Modulation of olfactory bulb tyrosine hydroxylase and catecholamine transporter mRNA by estrogen.

Since estrogen exerts wide ranging effects within the central nervous system, it is important to investigate the sites and actions of this gonadal steroid hormone at extra-hypothalamic locations. In the present report, the effects of estrogen upon…

APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus.

Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial…

Tags: 2005, 3' Untranslated Regions/genetics, Amino Acid, Animals, Antisense/*genetics/isolation & purification/*metabolism, Brain research. Molecular brain research, Conserved Sequence/genetics, DNA-Binding Proteins/genetics/*metabolism, Gainer Harold, Gene Expression Regulation/*genetics, Genetic/genetics, Genomic Imprinting/genetics, Glasgow Eric, Hypothalamo-Hypophyseal System/cytology/metabolism, Kruppel-Like Transcription Factors, Male, Messenger/genetics/metabolism, Molecular Sequence Data, Mutsuga Noriko, Neurons/*metabolism, Nucleic Acid, Protein Kinases/genetics/*metabolism, Rats, RNA, Ryu Seung-Lim, Sequence Homology, Sprague-Dawley, Supraoptic Nucleus/cytology/*metabolism, Transcription, Transcription Factors/genetics/*metabolism, Vasopressins/*metabolism, Water-Electrolyte Balance/genetics, Yamashita Mitsuo, Zhang Bing-Jun

Protein thiyl radical mediates S-glutathionylation of complex I.

Complex I is a critical site of O(2)(*-) production and the major host of reactive protein thiols in mitochondria. In response to oxidative stress, complex I protein thiols at the 51- and 75-kDa subunits are reversibly

Tags: *Oxidative Stress, 2012, Amino Acid Motifs, Amino Acid Sequence, Animals, Binding Sites, Cattle, Cell Line, Chen Chwen-Lih, Chen Jingfeng, Chen Yeong-Renn, Cyclic N-Oxides/chemistry/pharmacology, Cysteine/chemistry/*metabolism, Department of Integrative Medical Sciences, Electron Transport Complex I/chemistry/*metabolism, Free radical biology & medicine, Free Radical Scavengers/chemistry/pharmacology, Free Radicals/chemistry/*metabolism, Glutathione/chemistry/*metabolism, Green Kari B, Heart/enzymology/metabolism, Kang Patrick T, Mice, Mitochondria, Models, Molecular, Molecular Sequence Data, Muscle Cells/drug effects/metabolism, NEOMED College of Medicine, Onium Compounds/pharmacology, Peptide Fragments/chemistry, Peptide Mapping, Protein, Rats, Rotenone/pharmacology, Structural Homology, Superoxides/metabolism, Zhang Liwen

The role of the N-terminal and mid-region residues of substance P in regulating functional selectivity at the tachykinin NK1 receptor.

Previous studies have shown that tachykinin peptide ligands of the tachykinin NK1 receptor exhibit functional selectivity with respect to signal activation and desensitization. The differences are most dramatic between the naturally occurring…

Transcriptional regulation of human oxysterol 7alpha-hydroxylase by sterol response element binding protein.

Oxysterol 7alpha-hydroxylase (CYP7B1) metabolizes oxysterols, potent regulators of lipid homeostasis. Very little is known about transcriptional regulation of human CYP7B1. The present results indicate that sterol response element binding protein…

Calcium channel blockade attenuates angiotensin II-induced drinking in rats.

Lateral ventricular administration of angiotensin II (ANG II) produces potent dipsogenic effects in water-sated rats. ANG II seems to require functional voltage-gated calcium channels on neurons throughout circumventricular brain sites to exert its…

Tags: 1993, Amino Acid Sequence, Angiotensin II/*antagonists & inhibitors/pharmacology, Animals, Brain research bulletin, Calcagnetti D J, Calcium Channel Blockers/administration & dosage/*pharmacology, Dimethyl Sulfoxide/pharmacology, Dose-Response Relationship, Drinking Behavior/*drug effects, Drug, Injections, Intraventricular, Isradipine/pharmacology, Male, Molecular Sequence Data, Rats, Schechter M D, Sprague-Dawley

Tags: *Genes, 1991, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cytochromes b5/blood/*genetics, Gene Library, Genetic, Giordano S J, Humans, Liver/*metabolism, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Reticulocytes/*metabolism, RNA/blood/genetics/isolation & purification, Steggles A W

Browse Items (53 total)