Browse Items · NEOMED Bibliography Database

Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.

Oxysterol 7 alpha-hydroxylase catalyzes hydroxylation of oxysterols and neurosterols and plays a role in the alternative bile acid synthesis pathway. This gene is widely expressed in many organs and peripheral tissues and may protect tissues from the…

Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.

Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA…

Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.

A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol

Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).

The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion…

Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).

The cholesterol 7alpha-hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The…

Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4alpha (HNF4alpha).

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyses sterol side-chain oxidation of bile acid synthesis from cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27A1 gene…

Tags: *DNA-Binding Proteins, 2003, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Bile Acids and Salts/*pharmacology, Binding Sites/genetics, Cell Line, Chen Wenling, Chenodeoxycholic Acid/pharmacology, Chiang John Y L, Cholestanetriol 26-Monooxygenase, Cloning, Cultured, Cytoplasmic and Nuclear/genetics/metabolism, Department of Integrative Medical Sciences, DNA, DNA/chemistry/genetics, Dose-Response Relationship, Drug, gene, Gene Expression Regulation/drug effects, Genetic/*genetics, Hepatocyte Nuclear Factor 4, Humans, Luciferases/genetics/metabolism, Molecular, Molecular Sequence Data, Mutagenesis, Mutation, NEOMED College of Medicine, Phosphoproteins/genetics/*metabolism, Promoter Regions, Receptors, Recombinant Fusion Proteins/genetics/metabolism, Response Elements/genetics, Sequence Analysis, Site-Directed, Steroid Hydroxylases/*genetics, Transcription Factors/genetics/*metabolism, Transfection, Tumor Cells

Nuclear receptor-mediated repression of human cholesterol 7alpha-hydroxylase gene transcription by bile acids.

Hydrophobic bile acids strongly repressed transcription of the human cholesterol 7alpha-hydroxylase gene (CYP7A1) in the bile acid biosynthetic pathway in the liver. Farnesoid X receptor (FXR) repressed CYP7A1/Luc reporter activity in a transfection…

Tags: *Hydroxysteroid Dehydrogenases, *Membrane Glycoproteins, 2001, Animals, Bile Acids and Salts/*pharmacology, Caco-2 Cells, Carrier Proteins/genetics/physiology, Cell Line, Chen W, Chiang J Y, CHO Cells, Cholesterol 7-alpha-Hydroxylase/*genetics, Cricetinae, Cultured, Cytoplasmic and Nuclear/genetics/*physiology, Department of Integrative Medical Sciences, DNA-Binding Proteins/drug effects/genetics/physiology, DNA/metabolism, Embryo, Gene Expression/*drug effects, Genetic, Genetic/drug effects, Humans, Journal of lipid research, Kidney, Liver/metabolism, Luciferases/genetics, Mammalian, Messenger/analysis, NEOMED College of Medicine, Owsley E, Promoter Regions, Rats, Receptors, Recombinant Fusion Proteins/metabolism, Retinoic Acid/genetics/physiology, Retinoid X Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Stroup D, Taurocholic Acid/pharmacology, Transcription, Transcription Factors/drug effects/genetics/physiology, Transfection, Tumor Cells, Yang Y

Functional outcome after anal sphincter injury and treatment with mesenchymal stem cells.

This research demonstrates the regenerative effects of mesenchymal stem cells (MSCs) on the injured anal sphincter by comparing anal sphincter pressures following intramuscular and serial intravascular MSC infusion in a rat model of anal sphincter…

Gelucire-stabilized nanoparticles as a potential DNA delivery system.

Clinical viability of gene delivery systems has been greatly impacted by potential toxicity of the delivery systems. Recently, we reported the nanoparticle (NP) preparation process that employs biocompatible materials such as Gelucire(R) 44/14 and…

Aldo-keto reductase 1B7 is a target gene of FXR and regulates lipid and glucose homeostasis.

Aldo-keto reductase 1B7 (AKR1B7) is proposed to play a role in detoxification of by-products of lipid peroxidation. In this article, we show that activation of the nuclear receptor farnesoid X receptor (FXR) induces AKR1B7 expression in the liver and…

Surface clustering of metabotropic glutamate receptor 1 induced by long Homer proteins.

BACKGROUND: Metabotropic glutamate receptors (mGluRs) regulate neuronal excitability and synaptic strength. The group I mGluRs, mGluR1 and 5, are widespread in the brain and localize to post-synaptic sites. The Homer protein family regulates group I…

PKCalpha mediates acetylcholine-induced activation of TRPV4-dependent calcium influx in endothelial cells.

Transient receptor potential vanilloid channel 4 (TRPV4) is a polymodally activated nonselective cationic channel implicated in the regulation of vasodilation and hypertension. We and others have recently shown that cyclic stretch and shear stress…

Tags: 2011, Acetylcholine/*pharmacology, Adapala Ravi K, American journal of physiology. Heart and circulatory physiology, Animals, Bratz Ian N, Calcium Signaling/*drug effects, Carbazoles/pharmacology, Cells, Cultured, Department of Integrative Medical Sciences, Endothelial Cells/*drug effects/enzymology, Enzyme Activation, Inbred C57BL, Knockout, Male, Mesenteric Arteries/drug effects/enzymology, Meszaros J Gary, Mice, Mutation, NEOMED College of Medicine, Nitric Oxide Synthase Type III/metabolism, Phosphorylation, Protein Kinase C-alpha/genetics/*metabolism, Protein Kinase Inhibitors/pharmacology, Suzuki Makoto, Talasila Phani K, Tetradecanoylphorbol Acetate/pharmacology, Thodeti Charles K, Time Factors, Transfection, TRPV Cation Channels/*agonists/deficiency/genetics/metabolism, Vasodilation/*drug effects, Vasodilator Agents/*pharmacology, Zhang David X

Strain-dependent structural variants of herpes simplex virus type 1 ICP34.5 determine viral plaque size, efficiency of glycoprotein processing, and viral release and neuroinvasive disease potential.

The ability of certain strains of herpes simplex virus type 1 (HSV-1) to cause encephalitis or neuroinvasive disease in the mouse upon peripheral infection is dependent on a combination of activities of specific forms of viral proteins. The…

Rifampicin induction of CYP3A4 requires pregnane X receptor cross talk with hepatocyte nuclear factor 4alpha and coactivators, and suppression of small heterodimer partner gene expression.

Bile acids and drugs activate pregnane X receptor (PXR) to induce CYP3A4, which is the predominant cytochrome P450 enzyme expressed in the liver and intestine and plays a critical role in detoxifying bile acids and drugs, and protecting against…

Tags: 2006, Antibiotics, Antitubercular/*pharmacology, Blotting, Cell Line, Chiang John Y L, Chromatin/metabolism, Cytochrome P-450 CYP3A, Cytochrome P-450 Enzyme System/*biosynthesis, Cytoplasmic and Nuclear/*biosynthesis/*drug effects/genetics, Department of Integrative Medical Sciences, Drug metabolism and disposition: the biological fate of chemicals, Electrophoretic Mobility Shift Assay, Enzyme Induction/drug effects, Glutathione Transferase/metabolism, Hepatocyte Nuclear Factor 4/genetics/*metabolism, Hepatocytes/drug effects/metabolism, Humans, Immunoprecipitation, Li Tiangang, Messenger/biosynthesis, NEOMED College of Medicine, Plasmids/genetics, Pregnane X Receptor, Receptor Cross-Talk/drug effects, Receptors, Reverse Transcriptase Polymerase Chain Reaction, Rifampin/*pharmacology, RNA, Steroid/*drug effects/genetics, Transfection, Tumor, Western

A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene.

Prox1, an early specific marker for developing liver and pancreas in foregut endoderm has recently been shown to interact with alpha-fetoprotein transcription factor and repress cholesterol 7alpha-hydroxylase (CYP7A1) gene transcription. Using a…

Tags: *Gene Expression Regulation, 2006, Aged, Amino Acid Motifs, Bile Acids and Salts/metabolism, Cell Line, Cell Nucleus/metabolism, Cells, Chiang John Y L, Cholesterol 7-alpha-Hydroxylase/*chemistry/*genetics, Cultured/metabolism, Department of Integrative Medical Sciences, Enzymologic, Female, Genes, Genetic, Gluconeogenesis, Glutathione Transferase/metabolism, Hepatocyte Nuclear Factor 4/metabolism/*physiology, Hepatocytes/metabolism, Homeodomain Proteins/metabolism/*physiology, Humans, Immunoprecipitation, Li Tiangang, Liver/metabolism, Luciferases/metabolism, Male, Messenger/metabolism, Middle Aged, NEOMED College of Medicine, Phosphoenolpyruvate Carboxykinase (ATP)/metabolism, Plasmids/metabolism, Protein Structure, Reporter, Response Elements, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering/metabolism, Song Kwang-Hoon, Tertiary, The Journal of biological chemistry, Time Factors, Transcription, Transcriptional Activation, Transfection, Tumor Suppressor Proteins, Two-Hybrid System Techniques

Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.

Bcl-2 is an anti-apoptotic protein that has recently been shown to regulate other cellular functions. We previously reported that Bcl-2 regulates chondrocyte matrix gene expression, independent of its anti-apoptotic function. Here, we further…

Tags: *Gene Expression Regulation, 2005, Adenoviridae/genetics, Animals, Apoptosis, beta-Galactosidase/metabolism, Blotting, Butadienes/pharmacology, Caspase Inhibitors, Cell Differentiation, Cell Line, Chondrocytes/*metabolism, Collagen Type II/metabolism, Department of Anatomy & Neurobiology, Down-Regulation, Enzyme Inhibitors/pharmacology, Fibroblasts/metabolism, Fluorescence, Genetic, High Mobility Group Proteins/*metabolism, Horton Walter E Jr, Lac Operon, Luciferases/metabolism, MAP Kinase Kinase Kinases/*metabolism, McBurney Denise, Messenger/metabolism, Microscopy, Mitogen-Activated Protein Kinase 1/*metabolism, Mitogen-Activated Protein Kinase 3/*metabolism, NEOMED College of Medicine, NF-kappa B/metabolism, Nitriles/pharmacology, Phenotype, Phosphorylation, Promoter Regions, Protein Kinase C-alpha, Protein Kinase C/antagonists & inhibitors, Proteoglycans/metabolism, Proto-Oncogene Proteins c-bcl-2/*metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, RNA, Signal Transduction, Small Interfering/metabolism, SOX9 Transcription Factor, Sprague-Dawley, The Journal of biological chemistry, Time Factors, Transcription, Transcription Factors/*metabolism, Transfection, Western, Yagi Rieko

Prostate apoptosis response-4 enhances secretion of amyloid beta peptide 1-42 in human neuroblastoma IMR-32 cells by a caspase-dependent pathway.

Prostate apoptosis response-4 (Par-4) is a leucine zipper protein that promotes neuronal cell death in Alzheimer's disease (AD). Neuronal degeneration in AD may result from extracellular accumulation of amyloid beta peptide (Abeta) 1-42. To examine…

Par-4 is a synaptic protein that regulates neurite outgrowth by altering calcium homeostasis and transcription factor AP-1 activation.

Although Par-4 (prostate apoptosis response-4) is involved in initiation of neurodegenerative cascades associated with certain neurodegenerative disorders, normal physiological roles of Par-4 in neurons have remained elusive. It was recently reported…

Lipopolysaccharide supports maintaining the stemness of CD133(+) hepatoma cells through activation of the NF-kappaB/HIF-1alpha pathway.

Due to the existence of cancer stem cells (CSCs), persistence and relapse of human hepatocellular carcinoma (HCC) are common after treatment with existing anti-cancer therapies. Emerging evidence indicates that lipopolysaccharide (LPS) plays a…

Tags: *Cancer stem cells, *Lipopolysaccharide, *Plasticity, *Stemness maintenance, *Tumor microenvironment, 2016, AC133 Antigen/genetics/*metabolism, alpha Subunit/genetics/*metabolism, Animals, Antineoplastic Agents – Pharmacodynamics, Antineoplastic Agents/pharmacology, Body Weights and Measures, Cancer letters, Carcinoma, Cell Line, Cell Movement – Drug Effects, Cell Movement/drug effects, Cell Physiology, Cell Physiology – Drug Effects, Cell Proliferation/drug effects, Department of Integrative Medical Sciences, Drug Resistance, Gene Expression Regulation, Genes, Genetic Techniques, Han Zhi-Peng, Hepatocellular, Hepatocellular – Drug Therapy, Hepatocellular – Metabolism, Hepatocellular – Pathology, Hepatocellular/drug therapy/genetics/*metabolism/pathology, Humans, Hypoxia-Inducible Factor 1, Inbred BALB C, Jing Ying-Ying, Lai Fo-Bao, Li Rong, Li Xiao-Yong, Lipopolysaccharides – Pharmacodynamics, Lipopolysaccharides/*pharmacology, Liu Jing-Feng, Liu Wen-Ting, Liver Neoplasms, Liver Neoplasms – Drug Therapy, Liver Neoplasms – Metabolism, Liver Neoplasms – Pathology, Liver Neoplasms/drug therapy/genetics/*metabolism/pathology, Male, Mice, NEOMED College of Medicine, Neoplasm, Neoplasm Invasiveness, Neoplastic, Neoplastic Stem Cells/*drug effects/metabolism/pathology, NF-kappa B – Metabolism, NF-kappa B/*metabolism, Nude, Pan Xiao-Rong, Phenotype, Proteins, Proteins – Metabolism, RNA Interference, Shi Rong-Yu, Signal Transduction – Drug Effects, Signal Transduction/drug effects, Stem Cells – Drug Effects, Stem Cells – Metabolism, Stem Cells – Pathology, Time Factors, Transfection, Tumor Burden, Tumor Microenvironment, Wei Li-Xin, Wu Meng-Chao, Yang Xue, Yu Guo-Feng, Zeng Jian-Xing, Zhang Hang-Jie, Zhang Ping, Zhao Qiu-Dong

miR-217 regulates ethanol-induced hepatic inflammation by disrupting sirtuin

Ethanol-mediated injury, combined with gut-derived lipopolysaccharide (LPS), provokes generation of proinflammatory cytokines in Kupffer cells, causing hepatic inflammation. Among the mediators of these effects, miR-217 aggravates ethanol-induced…

The isolation and characterization of the human cytochrome b5 gene.

From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid…

Effects of bile acids and steroid/thyroid hormones on the expression of cholesterol 7 alpha-hydroxylase mRNA and the CYP7 gene in HepG2 cells.

The expression of cholesterol 7 alpha-hydroxylase mRNA levels in confluent HepG2 cultures was reduced by tauro- or glyco-conjugates of deoxycholate and chenodeoxycholate, but not by cholate. Ursodeoxycholates, on the other hand, stimulated the mRNA…

Promoter activity and regulation of the CYP4F2 leukotriene B(4) omega-hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells.

The human liver CYP4F2 gene (Accession No. AF221943) encodes a leukotriene B(4) omega-hydroxylase that metabolizes leukotriene B(4) (LTB(4)) to a less potent proinflammatory eicosanoid, 20-OH-LTB(4). We sequenced a 6.7-kb genomic fragment of the…

Osteoactivin regulates head and neck squamous cell carcinoma invasion by modulating matrix metalloproteases.

Nearly 60% of patients with head and neck squamous cell carcinoma (HNSCC) die of metastases or locoregional recurrence. Metastasis is mediated by cancer cell migration and invasion, which are in part dependent on extracellular matrix degradation by…

Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas.

Nearly 50% of patients with oral squamous cell carcinoma (OSCC) die of metastases or locoregional recurrence. Metastasis is mediated by cancer cell adhesion, migration, and invasion. Osteoactivin (OA) overexpression plays a role in metastases in…

A novel role for Bcl-2 associated-athanogene-1 (Bag-1) in regulation of the endoplasmic reticulum stress response in mammalian chondrocytes.

BAG-1 (Bcl-2 associated athanogene-1) is a multifunctional protein, linking cell proliferation, cell death, protein folding, and cell stress. In vivo, BAG-1 is expressed in growth plate and articular cartilage, and the expression of BAG-1 is…

Coordinate down-regulation of cartilage matrix gene expression in Bcl-2 deficient chondrocytes is associated with decreased SOX9 expression and decreased mRNA stability.

The anti-apoptotic protein Bcl-2 has been shown to function in roles unrelated to apoptosis in a variety of cell types. We have previously reported that loss of Bcl-2 expression alters chondrocyte morphology and modulates aggrecan expression via an…

MicroRNA-602 and microRNA-608 regulate sonic hedgehog expression via target sites in the coding region in human chondrocytes.

OBJECTIVE: Hedgehog (HH) signaling has recently been associated with cartilage degradation in osteoarthritis (OA). Because interleukin-1beta (IL-1beta) has been implicated as a principal instigator of OA, we sought to determine whether

Tags: 2015, Academic Medical Centers, Aged, Akhtar Nahid, Analysis of Variance, Animal, Animals, Anterior Cruciate Ligament/surgery, Arthritis & rheumatology (Hoboken, N.J.), Blotting, Cells, Chondrocytes – Physiology, Chondrocytes/drug effects/*metabolism/pathology, Cultured, Department of Anatomy & Neurobiology, Disease Models, Female, Funding Source, Gene Expression Regulation/genetics/*physiology, Haqqi Tariq M, Hedgehog Proteins/genetics/*metabolism, HEK293 Cells, Human, Humans, Immunohistochemistry, In Vitro Techniques, Interleukin-1beta/pharmacology, Knee/etiology/*metabolism/pathology, Makki Mohammad S, Male, Matrix Metalloproteinase 13/metabolism, MicroRNAs/genetics/*metabolism, Middle Age, Middle Aged, NEOMED College of Medicine, Ohio, Open Reading Frames/genetics/*physiology, Osteoarthritis, Osteoarthritis – Epidemiology, Osteoarthritis – Physiopathology, Polymerase Chain Reaction, Rabbits, Signal Transduction/genetics/physiology, T-Tests, Transfection, Up-Regulation/drug effects/genetics/physiology, Western

Browse Items (28 total)