Browse Items (17 total)
- Tags: Reverse Transcriptase Polymerase Chain Reaction
Nuclear receptor-mediated repression of human cholesterol 7alpha-hydroxylase gene transcription by bile acids.
Tags: *Hydroxysteroid Dehydrogenases, *Membrane Glycoproteins, 2001, Animals, Bile Acids and Salts/*pharmacology, Caco-2 Cells, Carrier Proteins/genetics/physiology, Cell Line, Chen W, Chiang J Y, CHO Cells, Cholesterol 7-alpha-Hydroxylase/*genetics, Cricetinae, Cultured, Cytoplasmic and Nuclear/genetics/*physiology, Department of Integrative Medical Sciences, DNA-Binding Proteins/drug effects/genetics/physiology, DNA/metabolism, Embryo, Gene Expression/*drug effects, Genetic, Genetic/drug effects, Humans, Journal of lipid research, Kidney, Liver/metabolism, Luciferases/genetics, Mammalian, Messenger/analysis, NEOMED College of Medicine, Owsley E, Promoter Regions, Rats, Receptors, Recombinant Fusion Proteins/metabolism, Retinoic Acid/genetics/physiology, Retinoid X Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Stroup D, Taurocholic Acid/pharmacology, Transcription, Transcription Factors/drug effects/genetics/physiology, Transfection, Tumor Cells, Yang Y
Black currant anthocyanins abrogate oxidative stress through Nrf2- mediated antioxidant mechanisms in a rat model of hepatocellular carcinoma.
Tags: *Phytotherapy, 2012, Alkylating Agents/toxicity, Animals, Anthocyanins/*therapeutic use, Antioxidants/therapeutic use, Barnes Kendra F, Bhatia Deepak, Bishayee Anupam, Blotting, Carcinoma, Current cancer drug targets, Darvesh Altaf S, Department of Pharmaceutical Sciences, Diet, Diethylnitrosamine/toxicity, Experimental/chemically induced/metabolism/*prevention & control, Glutathione Transferase, Haznagy-Radnai Erzsebet, Hepatocellular/chemically induced/metabolism/*prevention & control, Hohmann Judit, Immunoenzyme Techniques, Lipid Peroxidation/drug effects, Liver Neoplasms, Male, Messenger/genetics, NEOMED College of Pharmacy, NF-E2-Related Factor 2/genetics/*metabolism, Nitric Oxide Synthase Type II/genetics/metabolism, Oxidative Stress/*drug effects, Rats, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Ribes/*chemistry, RNA, Signal Transduction, Sprague-Dawley, Thoppil Roslin J, Tyrosine/analogs & derivatives/metabolism, Western
A class of genes in the HER2 regulon that is poised for transcription in breast cancer cell lines and expressed in human breast tumors.
Tags: *Gene Expression Profiling, *Gene Expression Regulation, 2015, Blotting, Breast Neoplasms/genetics/pathology, Cell Line, Char Jessica E, Chen Xin, ErbB-2/*genetics/metabolism, Franke Anna-Clara, Gene Regulatory Networks, Homeodomain Proteins/genetics/metabolism, Humans, Jia Zhenyu, Jones Frank E, McClelland Michael, MCF-7 Cells, Men Bozhao, Mercola Dan, Nanog Homeobox Protein, Neoplastic, Neoplastic Stem Cells/metabolism, Octamer Transcription Factor-3/genetics/metabolism, Oncotarget, Rahmatpanah Farah B, Receptor, Regulon/*genetics, Reverse Transcriptase Polymerase Chain Reaction, RNA Polymerase II/metabolism, SOXB1 Transcription Factors/genetics/metabolism, Tumor, Tumor Microenvironment/genetics, Western
Characterization of vascular endothelial growth factor (VEGF) in the uterine cervix over pregnancy: effects of denervation and implications for cervical ripening.
Tags: 2004, Animal/*metabolism, Animals, Cervical Ripening/*metabolism, Cervix Uteri/blood supply/innervation/*metabolism, Denervation, Department of Anatomy & Neurobiology, Down-Regulation, Enzyme-Linked Immunosorbent Assay, Female, Hattori Y, Immunohistochemistry, Jesmin S, Microcirculation, Mowa C N, NEOMED College of Medicine, Nitric Oxide Synthase Type III, Nitric Oxide Synthase/biosynthesis, Papka R, Phosphorylation, Pregnancy, Protein Isoforms/biosynthesis/metabolism, Protein-Serine-Threonine Kinases/biosynthesis, Proto-Oncogene Proteins c-akt, Proto-Oncogene Proteins/biosynthesis, Rats, Reverse Transcriptase Polymerase Chain Reaction, Sakuma I, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Togashi H, Usip S, Vascular Endothelial Growth Factor A/biosynthesis/*metabolism, Vascular Endothelial Growth Factor Receptor-2/biosynthesis, Wistar, Yoshioka M
Induction of vascular progenitor cells from endothelial cells stimulates coronary collateral growth.
Tags: *Collateral Circulation, *Coronary Circulation, 2012, Animal, Animals, Bailey Erin, Biomarkers/metabolism, Cell Differentiation, Cell Lineage, Cells, Chilian William M, Circulation research, Coronary Occlusion/genetics/metabolism/pathology/physiopathology/*surgery, Coronary Vessels/metabolism/pathology/*physiopathology, Cultured, Delucia Angelo, Department of Integrative Medical Sciences, Developmental, Disease Models, Endothelial Cells/metabolism/pathology/*transplantation, Enrick Molly, Epigenesis, Gene Expression Profiling, Gene Expression Regulation, Genetic, Guarini Giacinta, Induced Pluripotent Stem Cells/metabolism/*transplantation, Kolz Christopher L, Mice, Muscle, Myocytes, NEOMED College of Medicine, Neovascularization, Ohanyan Vahagn, Physiologic, Pung Yuh Fen, Rats, Regenerative Medicine/methods, Regional Blood Flow, Reverse Transcriptase Polymerase Chain Reaction, SCID, Smooth, Smooth Muscle/metabolism/pathology/*transplantation, Sprague-Dawley, Stevanov Kelly, Teratoma/metabolism/pathology, Time Factors, Transcription Factors/genetics/metabolism, Transduction, Vascular/metabolism/pathology/*physiopathology, Yin Liya
Rifampicin induction of CYP3A4 requires pregnane X receptor cross talk with hepatocyte nuclear factor 4alpha and coactivators, and suppression of small heterodimer partner gene expression.
Tags: 2006, Antibiotics, Antitubercular/*pharmacology, Blotting, Cell Line, Chiang John Y L, Chromatin/metabolism, Cytochrome P-450 CYP3A, Cytochrome P-450 Enzyme System/*biosynthesis, Cytoplasmic and Nuclear/*biosynthesis/*drug effects/genetics, Department of Integrative Medical Sciences, Drug metabolism and disposition: the biological fate of chemicals, Electrophoretic Mobility Shift Assay, Enzyme Induction/drug effects, Glutathione Transferase/metabolism, Hepatocyte Nuclear Factor 4/genetics/*metabolism, Hepatocytes/drug effects/metabolism, Humans, Immunoprecipitation, Li Tiangang, Messenger/biosynthesis, NEOMED College of Medicine, Plasmids/genetics, Pregnane X Receptor, Receptor Cross-Talk/drug effects, Receptors, Reverse Transcriptase Polymerase Chain Reaction, Rifampin/*pharmacology, RNA, Steroid/*drug effects/genetics, Transfection, Tumor, Western
Tags: 2017, Amino Acid Oxidoreductases/*genetics/metabolism, Animal, Animals, Balog Brian M, Borazjani Ali, Butler Robert S, Chemokine CCL7/genetics/metabolism, Chemokine CXCL12/genetics/metabolism, Couri Bruna M, Damaser Margot S, Delivery, Female, Female pelvic medicine & reconstructive surgery, Humans, Knockout, Kuang Mei, Lenis Andrew T, Mice, Models, Obstetric/*adverse effects/methods, Pelvic Organ Prolapse/*genetics/metabolism/pathology, Penn Marc S, Pregnancy, Reverse Transcriptase Polymerase Chain Reaction, Urethra/metabolism, Urinary Bladder/metabolism, Vagina/metabolism
A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene.
Tags: *Gene Expression Regulation, 2006, Aged, Amino Acid Motifs, Bile Acids and Salts/metabolism, Cell Line, Cell Nucleus/metabolism, Cells, Chiang John Y L, Cholesterol 7-alpha-Hydroxylase/*chemistry/*genetics, Cultured/metabolism, Department of Integrative Medical Sciences, Enzymologic, Female, Genes, Genetic, Gluconeogenesis, Glutathione Transferase/metabolism, Hepatocyte Nuclear Factor 4/metabolism/*physiology, Hepatocytes/metabolism, Homeodomain Proteins/metabolism/*physiology, Humans, Immunoprecipitation, Li Tiangang, Liver/metabolism, Luciferases/metabolism, Male, Messenger/metabolism, Middle Aged, NEOMED College of Medicine, Phosphoenolpyruvate Carboxykinase (ATP)/metabolism, Plasmids/metabolism, Protein Structure, Reporter, Response Elements, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering/metabolism, Song Kwang-Hoon, Tertiary, The Journal of biological chemistry, Time Factors, Transcription, Transcriptional Activation, Transfection, Tumor Suppressor Proteins, Two-Hybrid System Techniques
Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.
Tags: *Gene Expression Regulation, 2005, Adenoviridae/genetics, Animals, Apoptosis, beta-Galactosidase/metabolism, Blotting, Butadienes/pharmacology, Caspase Inhibitors, Cell Differentiation, Cell Line, Chondrocytes/*metabolism, Collagen Type II/metabolism, Department of Anatomy & Neurobiology, Down-Regulation, Enzyme Inhibitors/pharmacology, Fibroblasts/metabolism, Fluorescence, Genetic, High Mobility Group Proteins/*metabolism, Horton Walter E Jr, Lac Operon, Luciferases/metabolism, MAP Kinase Kinase Kinases/*metabolism, McBurney Denise, Messenger/metabolism, Microscopy, Mitogen-Activated Protein Kinase 1/*metabolism, Mitogen-Activated Protein Kinase 3/*metabolism, NEOMED College of Medicine, NF-kappa B/metabolism, Nitriles/pharmacology, Phenotype, Phosphorylation, Promoter Regions, Protein Kinase C-alpha, Protein Kinase C/antagonists & inhibitors, Proteoglycans/metabolism, Proto-Oncogene Proteins c-bcl-2/*metabolism, Rats, Reverse Transcriptase Polymerase Chain Reaction, RNA, Signal Transduction, Small Interfering/metabolism, SOX9 Transcription Factor, Sprague-Dawley, The Journal of biological chemistry, Time Factors, Transcription, Transcription Factors/*metabolism, Transfection, Western, Yagi Rieko
Substance P in the uterine cervix, dorsal root ganglia and spinal cord during pregnancy and the effect of estrogen on SP synthesis.
Tags: 2003, Afferent/metabolism, Amann R, Animals, Cervix Uteri/cytology/*metabolism, Department of Anatomy & Neurobiology, Down-Regulation, Estradiol/*analogs & derivatives/pharmacology, Estrogen/antagonists & inhibitors, Estrogens/*pharmacology, Female, Fulvestrant, Ganglia, Immunohistochemistry, In Situ Hybridization, Messenger/genetics/metabolism, Mowa C N, NEOMED College of Medicine, Neurons, Papka R, Peptides, Postpartum Period, Pregnancy/*metabolism, Radioimmunoassay, Rats, Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Spinal Cord/cytology/*metabolism, Spinal/cytology/*metabolism, Sprague-Dawley, Storey-Workley M, Substance P/*biosynthesis/genetics/metabolism, Up-Regulation, Usip S
Tags: 2006, Antiviral Agents/*pharmacology, Antiviral research, Bailey Erin, Blotting, Booth Tristan, Cell Line, Docherty John J, Dose-Response Relationship, Drug, Faith Seth A, Fibroblasts/virology, Herpesvirus 3, Human/*drug effects/physiology, Humans, Immediate-Early Proteins/biosynthesis, Messenger/biosynthesis, Resveratrol, Reverse Transcriptase Polymerase Chain Reaction, RNA, Stilbenes/*pharmacology, Sweet Thomas J, Trans-Activators/biosynthesis, Viral Envelope Proteins/biosynthesis, Viral Plaque Assay, Viral/biosynthesis, Virus Attachment/drug effects, Virus Inactivation/drug effects, Virus Replication/*drug effects, Western
Tags: 2006, Animals, Antiviral Agents/*pharmacology, Antiviral research, Bailey Erin, Booth Tristan, Cell Nucleus/chemistry, Cercopithecus aethiops, Cytoplasm/chemistry, DNA, Docherty John J, Electrophoretic Mobility Shift Assay, Faith Seth A, Fluorescence, Herpesvirus 1, Herpesvirus 2, Human/drug effects/genetics/*growth & development, Human/drug effects/genetics/*physiology, I-kappa B Proteins/metabolism, Messenger/biosynthesis, Microscopy, NF-kappa B/*metabolism, NF-KappaB Inhibitor alpha, Polymerase Chain Reaction, Resveratrol, Reverse Transcriptase Polymerase Chain Reaction, RNA, Stilbenes/*pharmacology, Sweet Thomas J, Transcription Factor RelA/metabolism, Vero Cells, Viral/biosynthesis, Virus Replication/*drug effects
Analysis of connective tissues by laser capture microdissection and reverse transcriptase-polymerase chain reaction.
Tags: *Microdissection, 2005, Analytical biochemistry, Animals, Cell Separation/*methods, Chondrocytes/chemistry/cytology, Connective Tissue Cells/chemistry/*cytology, Gene Expression Profiling/*methods, Growth Plate/cytology, Hillyer Jennifer, Inbred Strains, Jacquet Robin, Landis William J, Lasers, Messenger/analysis/*isolation & purification, Methods, Mice, Newborn, Reverse Transcriptase Polymerase Chain Reaction, RNA, Tibia/cytology
The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.
Tags: *Stress, 2011, Animals, Apoptosis/drug effects, Articular/cytology, Blotting, Cartilage, Cattle, Cells, Chondrocytes/cytology/drug effects/*metabolism, Cultured, Department of Anatomy & Neurobiology, DNA-Binding Proteins/genetics/metabolism, Dose-Response Relationship, Drug, Endoplasmic Reticulum/*metabolism, Extracellular Matrix/*metabolism, Glucose/pharmacology, Heat-Shock Proteins/genetics/metabolism, Horton Walter E Jr, Journal of cellular biochemistry, McBurney Denise L, NEOMED College of Medicine, Nugent Ashleigh E, Physiological, Proto-Oncogene Proteins c-bcl-2/genetics/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Thapsigargin/pharmacology, Time Factors, Transcription Factors/genetics/metabolism, Tunicamycin/pharmacology, Western
Modified aminosilane substrates to evaluate osteoblast attachment, growth, and gene expression in vitro.
Tags: *Cell Adhesion, *Cell Division, *Gene Expression, *Silanes/chemistry, 2006, Alkaline Phosphatase/metabolism, Animals, Base Sequence, Chick Embryo, Collagen/metabolism, DNA Primers, Jacquet R, Journal of biomedical materials research. Part A, Landis W J, Osteoblasts/*cytology/enzymology/metabolism, Reverse Transcriptase Polymerase Chain Reaction, RNA/isolation & purification/metabolism, Siperko L M
Bile acids and cytokines inhibit the human cholesterol 7 alpha-hydroxylase gene via the JNK/c-jun pathway in human liver cells.
Tags: 2006, Bile Acids and Salts/*metabolism, Cells, Chenodeoxycholic Acid/pharmacology, Chiang John Y L, Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism, Cultured, Cytokines/*metabolism, Department of Integrative Medical Sciences, Gene Expression Regulation, Genetic, Hepatocytes/*cytology/drug effects, Hepatology (Baltimore, Md.), Humans, Immunoblotting, In Vitro Techniques, Interleukin-1/pharmacology, Jahan Asmeen, Li Tiangang, Messenger/analysis, NEOMED College of Medicine, Probability, Proto-Oncogene Proteins c-jun/*metabolism, Reverse Transcriptase Polymerase Chain Reaction, RNA, Sensitivity and Specificity, Signal Transduction/genetics, Transcription
MicroRNA-9 promotion of interleukin-6 expression by inhibiting monocyte chemoattractant protein-induced protein 1 expression in interleukin-1beta-stimulated human chondrocytes.
Tags: 2015, Academic Medical Centers, Aged, Arthritis & rheumatology (Hoboken, N.J.), Articular/drug effects/*metabolism, Cartilage, Cells, Chondrocytes – Physiology, Chondrocytes/drug effects/*metabolism, Cultured, Department of Anatomy & Neurobiology, Enzyme-Linked Immunosorbent Assay, Funding Source, Gene Knockdown Techniques, Haqqi Tariq M, Haseeb Abdul, Hip/*genetics/metabolism, Human, Humans, Immunoblotting – Utilization, Immunoprecipitation, Interleukin-1beta/pharmacology, Interleukin-6/*genetics/metabolism, Interleukins – Physiology, Makki Mohammad S, Messenger/drug effects/*metabolism, MicroRNAs/drug effects/*genetics/metabolism, Middle Aged, NEOMED College of Medicine, Ohio, Osteoarthritis, Osteoarthritis – Complications, Osteoarthritis – Physiopathology, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleases/drug effects/*genetics/metabolism, RNA, T-Tests, Transcription Factors/drug effects/*genetics/metabolism