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Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.

Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA…

Peroxisome proliferator-activated receptor alpha (PPARalpha) and agonist inhibit cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription.

Fibrates are widely used hypolipidemic drugs that regulate the expression of many genes involved in lipid metabolism by activating the peroxisome proliferator-activated receptor alpha (PPARalpha). The objective of this study was to investigate the…

Tags: *DNA-Binding Proteins, *Regulatory Sequences, 2000, Animals, Anticholesteremic Agents/pharmacology, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Binding Sites, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics, Clofibric Acid/pharmacology, Cytoplasmic and Nuclear/*agonists, Department of Integrative Medical Sciences, Enzymologic, Gene Expression Regulation, Genes, Genetic, Hepatocyte Nuclear Factor 4, Humans, Journal of lipid research, Liver/metabolism, Marrapodi M, Models, NEOMED College of Medicine, Nucleic Acid, Peroxisome Proliferators/pharmacology, Phosphoproteins/metabolism, Promoter Regions, Protein Binding, Pyrimidines/pharmacology, Rats, Receptors, Reporter, Response Elements, Retinoic Acid/metabolism, Retinoid X Receptors, Species Specificity, Transcription, Transcription Factors/*agonists/metabolism, Transcriptional Activation

Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.

Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of…

Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.

We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two…

Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.

RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products…

Tags: 1992, Amino Acid Sequence, Animals, Base Sequence, Cytochromes b5/chemistry/*genetics, Giordano S J, Leukocytes/*chemistry, Liver/chemistry, Messenger/*chemistry, Molecular Sequence Data, Nucleic Acid, Nucleic Acid Hybridization, Polymerase Chain Reaction, Rabbits, Reticulocytes/*chemistry, RNA, SAAS bulletin, biochemistry and biotechnology, Sequence Homology, Steggles A W

Isolation and characterization of cDNA clones for human, bovine and rabbit liver cytochrome b5 mRNA's.

Transcriptional activation of the cholesterol 7alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors.

The gene encoding cholesterol 7alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the…

Tags: *Receptors, *Transcriptional Activation, 1998, Animals, Base Sequence, Bile Acids and Salts/biosynthesis, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, COUP Transcription Factor II, COUP Transcription Factors, Crestani M, Cultured, Cytoplasmic and Nuclear/*physiology, Department of Integrative Medical Sciences, DNA-Binding Proteins/metabolism, DNA/metabolism, Galli G, Genes, Genetic, Hepatocyte Nuclear Factor 4, Hormones/*physiology, Journal of lipid research, Luciferases/genetics, Molecular Sequence Data, Mutagenesis, NEOMED College of Medicine, Nucleic Acid, Oligonucleotide Probes/metabolism, Phosphoproteins/metabolism, Promoter Regions, Rats, Receptors, Repetitive Sequences, Reporter, Retinoic Acid/metabolism, Retinoid X Receptors, Sadeghpour A, Site-Directed, Steroid, Stroup D, Transcription Factors/metabolism, Tumor Cells

Intrastrain variants of herpes simplex virus type 1 isolated from a neonate with fatal disseminated infection differ in the ICP34.5 gene, glycoprotein processing, and neuroinvasiveness.

Two intrastrain variants of herpes simplex virus type 1 (HSV-1) were isolated from a newborn with fatal disseminated infection. A small-plaque-producing variant (SP7) was the predominant virus (\textgreater99%) in the brain, and a…

APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus.

Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial…

Tags: 2005, 3' Untranslated Regions/genetics, Amino Acid, Animals, Antisense/*genetics/isolation & purification/*metabolism, Brain research. Molecular brain research, Conserved Sequence/genetics, DNA-Binding Proteins/genetics/*metabolism, Gainer Harold, Gene Expression Regulation/*genetics, Genetic/genetics, Genomic Imprinting/genetics, Glasgow Eric, Hypothalamo-Hypophyseal System/cytology/metabolism, Kruppel-Like Transcription Factors, Male, Messenger/genetics/metabolism, Molecular Sequence Data, Mutsuga Noriko, Neurons/*metabolism, Nucleic Acid, Protein Kinases/genetics/*metabolism, Rats, RNA, Ryu Seung-Lim, Sequence Homology, Sprague-Dawley, Supraoptic Nucleus/cytology/*metabolism, Transcription, Transcription Factors/genetics/*metabolism, Vasopressins/*metabolism, Water-Electrolyte Balance/genetics, Yamashita Mitsuo, Zhang Bing-Jun

Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.

Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This…

The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.

This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that…

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