Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.

Title

Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.

Creator

Signs S A; Jacquet R

Publisher

Molecular and cellular biochemistry

Date

1994
1994-10

Description

Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This regimen resulted in the development of tolerance and dependence upon ethanol. RNA isolated from the ethanol-dependent rat brains was used to construct a cDNA library. One cDNA was identified that hybridized to a mRNA which increased in rat brain during the ethanol treatment. Sequence analysis of the cDNA indicated that it recognized a mRNA in rat brain which was very similar to that which encodes the 18 kDa subunit of canine signal peptidase. The rat signal peptidase mRNA was observed to increase in brain nearly 2-fold within 48 h after the initiation of ethanol treatment. Ethanol did not significantly alter beta-actin mRNA levels during the treatment period. These results support the existence of an ethanol-responsive signal peptidase mRNA in rat brain.

Subject

*Membrane Proteins; *Serine Endopeptidases; Alcoholism/*enzymology; Amino Acid; Amino Acid Sequence; Animals; Base Sequence; Blotting; Brain/*enzymology; Complementary; DNA; Endopeptidases/*biosynthesis/chemistry/genetics; Ethanol/*pharmacology; Male; Messenger/genetics/metabolism; Molecular Sequence Data; Northern; Nucleic Acid; Rats; RNA; Sequence Homology; Sprague-Dawley; Substance Withdrawal Syndrome/enzymology

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

21–26

Issue

1

Volume

139

Citation

Signs S A; Jacquet R, “Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.,” NEOMED Bibliography Database, accessed June 19, 2021, https://neomed.omeka.net/items/show/3142.

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