The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.

Title

The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.

Creator

Cristiano R J; Giordano S J; Steggles A W

Publisher

Genomics

Date

1993
1993-08

Description

This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that described for human and rabbit b5. All of the splicing junctions conform to the GT-AG consensus rule. The 5' flanking sequence has no obvious TATA box, two CAAT boxes, and contains several G:C-rich SpI motifs indicative of a house-keeping gene. In reticulocyte mRNA we found evidence for a transcribed b5 pseudogene, but could not detect sequences coding for the soluble form of b5. We conclude that the soluble form of b5 is derived from the membrane-bound b5 by a post-translational mechanism.

Subject

*Pseudogenes; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cattle/*genetics; Cytochromes b5/*genetics; DNA/genetics/isolation & purification; Exons; Gene Expression; Genetic; Genomic Library; Humans; Introns; Messenger/biosynthesis/metabolism; Molecular Sequence Data; Nucleic Acid; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rabbits; Restriction Mapping; Reticulocytes/metabolism; RNA; RNA Precursors/chemistry/metabolism; Sequence Homology; Southern; Transcription

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

348–354

Issue

2

Volume

17

Citation

Cristiano R J; Giordano S J; Steggles A W, “The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.,” NEOMED Bibliography Database, accessed June 20, 2021, https://neomed.omeka.net/items/show/3116.

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