A L.E.A.P.S. heteroconjugate vaccine containing a T cell epitope from HSV-1 glycoprotein D elicits Th1 responses and protection.

A L.E.A.P.S. heteroconjugate vaccine containing a T cell epitope from HSV-1 glycoprotein D elicits Th1 responses and protection.

Goel N; Rong Q; Zimmerman D; Rosenthal K S

Vaccine

2003

2003-10

The L.E.A.P.S. heteroconjugate vaccine antigen (JgD), composed of a T cell epitope from glycoprotein D (gD(8-23)) of herpes simplex virus (HSV) linked with a peptide sequence from beta-2-microglobulin (aa38-50), elicited protection against lethal intraperitoneal (IP) challenge and prevented disease signs in most, and limited disease progression, for the rest of BALB/c mice challenged in the epidermal abrasion-zosteriform spread mouse infection model. JgD elicited a Th1 response in vaccinated mice as indicated by delayed type hypersensitivity (DTH) responses to HSV antigen, and gD and virion specific antibodies with an IgG2a/IgG1 \textgreater1. Vaccination with the JgD peptide delayed the onset of disease signs, reduced severity of the disease and reduced mortality rates in mice with different MHC backgrounds as compared to their respective control mice. CD8 cells were demonstrated as important for initiation of the immune response to JgD and CD4 cells and interferon gamma (IFN-gamma) for delivering immune protection in BALB/c mice, as indicated in monoclonal antibody ablation studies. JgD, and other

Amino Acid Sequence; Animals; CD4-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/immunology; Conjugate/immunology; Delayed/immunology; Enzyme-Linked Immunosorbent Assay; Epitopes/*immunology; Female; Herpes Simplex Virus Vaccines/*immunology; Herpes Simplex/pathology/prevention & control; Herpesvirus 1; Human/*immunology; Hypersensitivity; Inbred BALB C; Interferon-gamma/biosynthesis; Lymphocyte Count; Mice; Molecular Sequence Data; Peptides/immunology; Th1 Cells/*immunology; Vaccines; Viral Envelope Proteins/*immunology

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