Using O2 to probe membrane immersion depth by 19F NMR.

Title

Using O2 to probe membrane immersion depth by 19F NMR.

Creator

Prosser R S; Luchette P A; Westerman P W

Publisher

Proceedings of the National Academy of Sciences of the United States of America

Date

2000
2000-08

Description

A fluorinated detergent, CF(3)(CF(2))(5)C(2)H(4)-O-maltose, was reconstituted into a lipid bilayer model membrane system to demonstrate the feasibility of determining solvent accessibility and membrane immersion depth of each fluorinated group by (19)F NMR. Apolar oxygen, which is known to partition with an increasing concentration gradient toward the hydrophobic membrane interior, exhibits a range of paramagnetic relaxation effects on (19)F nuclei, depending on its depth in the membrane. This effect, which is predominately associated with spin-lattice relaxation rates (R(1)) and chemical shifts, can be amplified greatly with minimal line broadening by increasing the partial pressure of O(2) at least 100-fold (i.e., P(O(2)) greater than 20 bar). The differences of longitudinal relaxation rates at 20 bar of oxygen pressure to those under ambient pressure (R(1)(20bar) - R(1)(0)) are largest for those fluorine groups expected to be most deeply buried in the membrane bilayer. This result contrasts with the reverse trend, which is observed on addition of a membrane surface-associated paramagnetic species, 4-(N,N-dimethyl-N-hexadecyl) ammonium-2,2,6,6-tetramethylpiperidine-1-oxyl iodide (CAT-16) at ambient pressures. Thus, differential relaxation rates may be observed in (19)F-labeled membrane-associated molecules resulting from the addition of apolar oxygen under high pressure. The results demonstrate that the degree of solvent accessibility and membrane immersion depth of specific fluorinated species in membrane-associated macromolecules can be probed by (19)F NMR.

Subject

*Hydrocarbons; *Oxygen; Biological; Cyclic N-Oxides; Dimyristoylphosphatidylcholine/*chemistry; Fluorinated; Fluorine; Lipid Bilayers/*chemistry; Magnetic Resonance Spectroscopy/*methods; Maltose/*analogs & derivatives; Models; Phospholipid Ethers/*chemistry; Pressure; Spin Labels

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

9967–9971

Issue

18

Volume

97

Citation

Prosser R S; Luchette P A; Westerman P W, “Using O2 to probe membrane immersion depth by 19F NMR.,” NEOMED Bibliography Database, accessed July 18, 2024, https://neomed.omeka.net/items/show/4057.