Rat alpha(2)-macroglobulin inhibits NGF-promoted neurite outgrowth, TrK phosphorylation, and gene expression of pheochromocytoma PC12 cells.

Title

Rat alpha(2)-macroglobulin inhibits NGF-promoted neurite outgrowth, TrK phosphorylation, and gene expression of pheochromocytoma PC12 cells.

Creator

Lee P G; Koo P H

Publisher

Journal of neuroscience research

Date

1999
1999-09

Description

Rat alpha-1-macroglobulin (alpha(1)M) and alpha-2-macroglobulin (alpha(2)M) are murine homologs of human alpha(2)M, and rat alpha(2)M is generally known as an acute-phase protein. Monoamine-activated forms of human alpha(2)M have been shown to inhibit various neuronal functions, but the effect of rat alpha(1)M and acute-phase alpha(2)M on neurons is largely unknown. In this report, rat serotonin-activated alpha(2)M (5HT-alpha(2)M) has been demonstrated to inhibit nerve growth factor (NGF)-promoted neurite extension in pheochromocytoma PC12 cells, and we investigated its possible mechanism of action including its effect on NGF-promoted signal transduction and gene expression in these cells. Especially in the absence of NGF, 5HT-alpha(2)M was found to bind to TrkA (the high-affinity receptor for NGF) much better than normal alpha(2)M (N-alpha(2)M).

Subject

Animals; Rats; Gene Expression Regulation; Phosphorylation; PC12 Cells; Proto-Oncogene Proteins c-jun/genetics; Adrenal Gland Neoplasms/metabolism; alpha-Macroglobulins/*pharmacology; Nerve Growth Factors/*antagonists & inhibitors; Neurites/*drug effects; Pheochromocytoma/metabolism; Sprague-Dawley; Receptor; Neoplastic/*drug effects; trkA/*metabolism

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

872–883

Issue

6

Volume

57

Citation

Lee P G; Koo P H, “Rat alpha(2)-macroglobulin inhibits NGF-promoted neurite outgrowth, TrK phosphorylation, and gene expression of pheochromocytoma PC12 cells.,” NEOMED Bibliography Database, accessed May 16, 2021, https://neomed.omeka.net/items/show/5504.

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