The effects of aging on mu and delta opioid receptors in the spinal cord of Fischer-344 rats.

Title

The effects of aging on mu and delta opioid receptors in the spinal cord of Fischer-344 rats.

Creator

Hoskins D L; Gordon T L; Crisp T

Publisher

Brain research

Date

1998
1998-04

Description

Previous research has demonstrated that the antinociceptive efficacy of opioids decreases with advancing age. This study utilized radioligand binding techniques to determine if this decline is due to a change in the receptor density (Bmax) and/or affinity (measured as Kd) of the mu (mu) and/or delta (delta) opioid receptors in the spinal cord with advancing age. Saturation binding analysis with [3H][d-Ala2,N-methyl-Phe4,Gly5-ol]enkephalin (DAMGO: a mu-opioid selective agonist) and [3H]naltrindole (a delta-opioid selective antagonist) revealed no age-related changes in Bmax for either the mu or delta-opioid receptors. The Kd value for naltrindole was likewise unaffected by age. The Kd value for DAMGO however, was significantly higher in the aged group as compared with the young and mature groups, indicating a decreased affinity of spinal mu-opioid receptors for DAMGO.

Subject

Aging/*metabolism; Ala(2)-MePhe(4)-Gly(5)-; Animals; delta/antagonists & inhibitors/*metabolism; Enkephalin; Enkephalins/metabolism; Inbred F344; Male; mu/agonists/*metabolism; Naltrexone/analogs & derivatives/metabolism; Narcotic Antagonists/metabolism; Opioid; Radioligand Assay; Rats; Receptors; Spinal Cord/*metabolism

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

299–302

Issue

1

Volume

791

Citation

Hoskins D L; Gordon T L; Crisp T, “The effects of aging on mu and delta opioid receptors in the spinal cord of Fischer-344 rats.,” NEOMED Bibliography Database, accessed August 5, 2021, https://neomed.omeka.net/items/show/3840.

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