Selective Targeting of Heme Protein in Cytochrome P450 and Nitric Oxide Synthase by Diphenyleneiodonium.

Selective Targeting of Heme Protein in Cytochrome P450 and Nitric Oxide Synthase by Diphenyleneiodonium.

Szilagyi John T; Mishin Vladimir; Heck Diane E; Jan Yi-Hua; Aleksunes Lauren M; Richardson Jason R; Heindel Ned D; Laskin Debra L; Laskin Jeffrey D

Toxicological sciences : an official journal of the Society of Toxicology

2016

2016-05

Cytochrome P450 (CYP) enzymes mediate mixed-function oxidation reactions important in drug metabolism. The aromatic heterocyclic cation, diphenyleneiodonium (DPI), binds flavin in cytochrome P450 reductase and inhibits

*cytochrome P450; *flavoenzymes; *heme; *nitric oxide synthase; *reactive oxygen species; Animals; Cytochrome P-450 Enzyme Inhibitors/*pharmacology; Cytochrome P-450 Enzyme System/*metabolism; Dose-Response Relationship; Drug; Heme/*antagonists & inhibitors/metabolism; Humans; Liver/drug effects/enzymology; Mice; Microsomes; Nitric Oxide Synthase Type II/*antagonists & inhibitors/metabolism; Nitric Oxide/metabolism; Onium Compounds/*pharmacology; Rats; Recombinant Proteins/metabolism; Sprague-Dawley; Time Factors

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