Ultrastructural examination of the corticocollicular pathway in the guinea pig: a study using electron microscopy, neural tracers, and GABA immunocytochemistry.

Title

Ultrastructural examination of the corticocollicular pathway in the guinea pig: a study using electron microscopy, neural tracers, and GABA immunocytochemistry.

Creator

Nakamoto Kyle T; Mellott Jeffrey G; Killius Jeanette; Storey-Workley Megan E; Sowick Colleen S; Schofield Brett R

Publisher

Frontiers in neuroanatomy

Date

2013
2013

Description

Projections from auditory cortex (AC) can alter the responses of cells in the inferior colliculus (IC) to sounds. Most IC cells show excitation and inhibition after stimulation of the AC. AC axons release glutamate and excite their targets, so inhibition is presumed to result from cortical activation of GABAergic IC cells that inhibit other IC cells via local projections. However, it is not known whether cortical axons contact GABAergic IC cells directly. We labeled corticocollicular axons by injecting fluorescent dextrans into the AC in guinea pigs. We visualized the tracer with diaminobenzidine and processed the tissue for electron microscopy. We identified presumptive GABAergic profiles with post-embedding anti-GABA immunogold histochemistry on ultrathin sections. We identified dextran-labeled cortical boutons in the IC and identified their postsynaptic targets according to morphology (e.g., spine, dendrite) and

Subject

auditory cortex; bouton classification; corticofugal pathways; inferior colliculi; synaptic targets; ultrastructural variations

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

13–13

Volume

7

Citation

Nakamoto Kyle T; Mellott Jeffrey G; Killius Jeanette; Storey-Workley Megan E; Sowick Colleen S; Schofield Brett R, “Ultrastructural examination of the corticocollicular pathway in the guinea pig: a study using electron microscopy, neural tracers, and GABA immunocytochemistry.,” NEOMED Bibliography Database, accessed May 5, 2021, https://neomed.omeka.net/items/show/5124.

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