Cholesterol-Peptide Hybrids to Form Liposome-Like Vesicles for Gene Delivery
Title
Cholesterol-Peptide Hybrids to Form Liposome-Like Vesicles for Gene Delivery
Creator
Tang Q; Cao B; Wu H Y; Cheng G
Publisher
PLOS ONE
Date
2013
2013-01
Description
In this paper, four amphiphilic cholesterol-peptide conjugates (Ch-R5H5, Ch-R3H3, Ch-R5 and Ch-R5) were designed and synthesized, and their properties in gene delivery were evaluated in vitro with an aim of developing more efficient gene delivery carriers. These amphiphilic cholesterol-peptide conjugates are composed of hydrophobic cholesterol and positively charged peptides. They were able to self-assemble into micelles at low concentrations and their critical micelle concentrations in phosphate buffered saline (pH 7.4) are <= 85 mu g/mL. Amphiphilic cholesterol-peptide conjugates condensed DNA more efficiently than a hydrophilic cationic oligoarginine (R10) peptide with no hydrophobic segment. Their transfection efficiencies were at least two orders of magnitude greater than that of R10 peptide in HEK-293 cells. Moreover, the introduction of histidine residues in cholesterol-peptide conjugates led to higher gene expression efficiency compared with cholesterol-peptides without histidine (Ch-R5 and Ch-R3), and the luciferase expression level was comparable or even higher than that induced by PEI at its optimal N/P ratio. In particular, Ch-R5H5 condensed DNA into smaller nanoparticles than Ch-R3H3 at higher N/P ratios, and the minimum size of Ch-R5H5/DNA complexes was 180 nm with zeta potential of 23 mV, achieved at the N/P ratio of 30. This liposome-like vesicle may be a promising gene delivery carrier for intravenous therapy.
Subject
condensation; dna; histidine; nanoparticles; oligopeptides; polymers; prospects; Science & Technology - Other Topics; systems; therapy; vectors
Identifier
Format
Journal Article
URL Address
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Rights
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Pages
8-8
Issue
1
Volume
8
Citation
Tang Q; Cao B; Wu H Y; Cheng G, “Cholesterol-Peptide Hybrids to Form Liposome-Like Vesicles for Gene Delivery,” NEOMED Bibliography Database, accessed May 8, 2024, https://neomed.omeka.net/items/show/7281.