Inhibition of rat brain pyruvate dehydrogenase by thiamine analogs.

Title

Inhibition of rat brain pyruvate dehydrogenase by thiamine analogs.

Creator

Kandiko C T; Smith D; Yamamoto B K

Publisher

Biochemical pharmacology

Date

1988
1988-11

Description

The effects of thiamine thiazolone (TT) and thiamine thiazolone pyrophosphate (TTPP) on the in vitro and in vivo inhibition of pyruvate dehydrogenase complex (PDHC) from rat cortex and hippocampus were characterized. TTPP decreased PDHC activity in vitro but had no effect in vivo following its direct chronic administration via osmotic mini-pumps into the brains of behaving rats. In contrast, TT had no direct effect in vitro following a differential centrifugation purification of the mitochondrial PDHC fraction, but decreased PDHC activity in vivo. Additional experiments demonstrated that the cytosolic fraction converted TT to TTPP which, in turn, inhibited PDHC in vitro. A mechanism is proposed to explain these effects that is consistent with a non-competitive inhibition of brain PDHC by TTPP.

Subject

Male; Animals; Rats; Kinetics; Brain/*enzymology; Cerebral Cortex/drug effects/enzymology; Hippocampus/drug effects/enzymology; Pyruvate Dehydrogenase Complex/*antagonists & inhibitors; Thiamine Pyrophosphate/analogs & derivatives/pharmacology; Thiamine/*analogs & derivatives/pharmacology; Inbred Strains

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

4375–4380

Issue

22

Volume

37

Citation

Kandiko C T; Smith D; Yamamoto B K, “Inhibition of rat brain pyruvate dehydrogenase by thiamine analogs.,” NEOMED Bibliography Database, accessed August 4, 2021, https://neomed.omeka.net/items/show/5471.

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