Bone marrow SSEA1+ cells support the myocardium in cardiac pressure overload.

Title

Bone marrow SSEA1+ cells support the myocardium in cardiac pressure overload.

Creator

Finan Amanda; Sopko Nikolai; Dong Feng; Turturice Ben; Kiedrowski Matthew; Penn Marc S

Publisher

PloS one

Date

2013
1905-07

Description

RATIONALE: Stage specific embryonic antigen 1+ (SSEA1+) cells have been described as the most primitive mesenchymal progenitor cell in the bone marrow. Cardiac injury mobilizes SSEA1+ cells into the peripheral blood but their in vivo function has not been characterized. OBJECTIVE: We generated animals with chimeric bone marrow to determine the fate and function of bone marrow SSEA1+ cells in response to acute cardiac pressure overload. METHODS AND RESULTS: Lethally irradiated mice were transplanted with normal bone marrow where the wild-type SSEA1+ cells were replaced with green fluorescent protein (GFP) SSEA1+ cells. Cardiac injury was induced by trans-aortic constriction (TAC). We identified significant GFP+ cell engraftment into the myocardium after TAC. Bone marrow GFP+ SSEA1 derived cells acquired markers of endothelial lineage, but did not express markers of c-kit+ cardiac progenitor cells. The function of bone marrow SSEA1+ cells after TAC was determined by transplanting lethally irradiated mice with bone marrow depleted of SSEA1+ cells (SSEA1-BM). The cardiac function of SSEA1-BM mice declined at a greater rate after TAC compared to their complete bone marrow transplant counterparts and was associated with decreased bone marrow cell engraftment and greater vessel rarefication in the myocardium. CONCLUSIONS: These results provide evidence for the recruitment of endogenous bone marrow SSEA1+ cells to the myocardium after TAC. We demonstrate that, in vivo, bone marrow SSEA1+ cells have the differentiation potential to acquire endothelial lineage markers. We also show that bone marrow SSEA1+ deficiency is associated with a reduced compensatory capacity to cardiac pressure overload, suggesting their importance in cardiac homeostasis. These data demonstrate that bone marrow SSEA1+ cells are critical for sustaining vascular density and cardiac repair to pressure overload.

Subject

Male; Animals; Mice; Mesenchymal Stem Cells/cytology/metabolism; Ventricular Remodeling; *Bone Marrow Transplantation; Bone Marrow Cells/cytology/*metabolism; Cell Tracking; Lewis X Antigen/*metabolism; Myocardium/cytology/*metabolism/pathology; Myocytes; Cardiac/cytology/metabolism

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

e68528–e68528

Issue

7

Volume

8

Citation

Finan Amanda; Sopko Nikolai; Dong Feng; Turturice Ben; Kiedrowski Matthew; Penn Marc S, “Bone marrow SSEA1+ cells support the myocardium in cardiac pressure overload.,” NEOMED Bibliography Database, accessed May 5, 2021, https://neomed.omeka.net/items/show/4944.

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