Alterations in gene array patterns in dendritic cells from aged humans.

Title

Alterations in gene array patterns in dendritic cells from aged humans.

Creator

Cao Jia-ning; Agrawal Anshu; Sharman Edward; Jia Zhenyu; Gupta Sudhir

Publisher

PloS one

Date

2014
1905-7

Description

Dendritic cells (DCs) are major antigen-presenting cells that play a key role in initiating and regulating innate and adaptive immune responses. DCs are critical mediators of tolerance and immunity. The functional properties of DCs decline with age. The purpose of this study was to define the age-associated molecular changes in DCs by gene array analysis using Affymatrix GeneChips. The expression levels of a total of 260 genes (1.8%) were significantly different (144 down-regulated and 116 upregulated) in monocyte-derived DCs (MoDCs) from aged compared to young human donors. Of the 260 differentially expressed genes, 24% were down-regulated by more than 3-fold, suggesting that a large reduction in expression occurred for a notable number of genes in the aged. Our results suggest that the genes involved in immune response to pathogens, cell migration and T cell priming display significant age-related changes. Furthermore, downregulated genes involved in cell cycle arrest and DNA replication may play a critical role in aging-associated genetic instability. These changes in gene expression provide molecular based evidence for age-associated functional abnormalities in human DCs that may be responsible for the defects in adaptive immunity observed in the elderly.

Subject

Adult; Female; Humans; Male; Aged; Young Adult; Immunity; Cluster Analysis; Age Factors; Gene Expression Regulation; *Gene Expression Profiling; *Transcriptome; Adaptive Immunity/genetics; Antigen-Presenting Cells/immunology/metabolism; Dendritic Cells/immunology/*metabolism; G1 Phase; Healthy Volunteers; 80 and over; Innate/genetics

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

e106471–e106471

Issue

9

Volume

9

Citation

Cao Jia-ning; Agrawal Anshu; Sharman Edward; Jia Zhenyu; Gupta Sudhir, “Alterations in gene array patterns in dendritic cells from aged humans.,” NEOMED Bibliography Database, accessed November 29, 2022, https://neomed.omeka.net/items/show/4949.

Social Bookmarking