The opposing effects of retinoic acid and phorbol esters converge to a common response element in the promoter of the rat cholesterol 7 alpha-hydroxylase gene (CYP7A).

Title

The opposing effects of retinoic acid and phorbol esters converge to a common response element in the promoter of the rat cholesterol 7 alpha-hydroxylase gene (CYP7A).

Creator

Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y

Publisher

Biochemical and biophysical research communications

Date

1996
1996-08

Description

The activity of the rat CYP7A/luciferase reporter gene was increased five-fold by all-trans retinoic acid (atRA) or 9-cis retinoic acid (9cRA) in transient transfection assay in HepG2 cells. Cotransfection with retinoid X receptor (RXR) stimulated the promoter activity in the absence of ligand, however, addition of atRA inhibited the transcriptional activity. Cotransfection with retinoic acid receptor (RAR) did not have much effect on CYP7A promoter activity. The CYP7A promoter, when linked upstream to the SV40/ luciferase reporter gene, strongly repressed the phorbol 12-myristate 13-acetate (PMA)-stimulated SV40/ luciferase reporter gene activity. The regions conferring the effects of RA and PMA were mapped to nt-176/ -117 and nt-148/-129, respectively. Several direct repeats of hormone response element (AGTTCA) in this region are required for RA response.

Subject

*Promoter Regions; Animals; Base Sequence; Cell Line; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; DNA Primers; Genetic; Molecular Sequence Data; Mutagenesis; Rats; Signal Transduction; Tetradecanoylphorbol Acetate/*pharmacology; Tretinoin/*pharmacology

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

585–592

Issue

2

Volume

225

Citation

Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y, “The opposing effects of retinoic acid and phorbol esters converge to a common response element in the promoter of the rat cholesterol 7 alpha-hydroxylase gene (CYP7A).,” NEOMED Bibliography Database, accessed February 25, 2021, https://neomed.omeka.net/items/show/3104.

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