Methods and insights from the characterization of osteoprogenitor cells of bats (Mammalia: Chiroptera).

Title

Methods and insights from the characterization of osteoprogenitor cells of bats (Mammalia: Chiroptera).

Creator

Ball H C; Moussa F M; Mbimba T; Orman R; Safadi F F; Cooper L N

Publisher

Stem cell research

Date

2016
2016-07

Description

Osteoprogenitor cells contribute to the development and maintenance of skeletal tissues. Bats are unique model taxa whose cellular processes are poorly understood, especially in regards to skeletal biology. Forelimb bones of bats, unlike those of terrestrial mammals, bend during flight and function in controlled deformation. As a first step towards understanding the molecular processes governing deposition of this flexible bone matrix, we provide the first method for isolation and differentiation of cell populations derived from the bone marrow and cortical bone of bats, and compare results with those harvested from C57BL/6J mice. Osteogenic capacity of these cells was assessed via absolute quantitative real-time PCR (qPCR) and through quantification of in vitro mineral deposition. Results indicate the differentiated bone cells of bats display significantly lower gene expression of known osteogenic markers (Runt-related transcription factor (RUNX2), osteocalcin (BGLAP) and osterix (SP7)), and deposit a less-mineralized matrix compared with murine controls. By characterizing the in vitro performance of osteoprogenitor cells throughout differentiation and matrix production, this study lays the ground work for in vitro manipulations of bat stem and osteoprogenitor cells and extends our understanding of the cellular diversity across mammals that occupy different habitats.

Subject

Animals; Bone Marrow Cells/cytology; Cell Differentiation/genetics; Cell Proliferation; Cells; Cellular Reprogramming; Chiroptera; Core Binding Factor Alpha 1 Subunit/genetics/metabolism; Cultured; Inbred C57BL; Mice; Osteoblasts/cytology/*metabolism; Osteocalcin/genetics/metabolism; Osteogenesis/*genetics; Real-Time Polymerase Chain Reaction; Stem Cells/*cytology/metabolism; Transcription Factors/genetics/metabolism

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

54–61

Issue

1

Volume

17

Citation

Ball H C; Moussa F M; Mbimba T; Orman R; Safadi F F; Cooper L N, “Methods and insights from the characterization of osteoprogenitor cells of bats (Mammalia: Chiroptera).,” NEOMED Bibliography Database, accessed July 24, 2021, https://neomed.omeka.net/items/show/3782.

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