Aflatoxin B1 metabolism by 3-methylcholanthrene-induced hamster hepatic cytochrome P-450s.

Aflatoxin B1 metabolism by 3-methylcholanthrene-induced hamster hepatic cytochrome P-450s.

Lai T S; Chiang J Y

Journal of biochemical toxicology

1990

1905-06

We have studied the activation of aflatoxin B1 by hamster liver microsomes and purified hamster cytochrome P-450 isozymes using a umu mutagen test. The hamster liver microsomes or S-9 fractions were much more active than rat liver microsomes or S-9 fractions in the activation of umu gene expression by aflatoxin B1 metabolites. 3-Methyl-cholanthrene treatment increased aflatoxin B1 activation by hamster liver microsomes. Two major 3-methylcholanthrene-inducible cytochrome

Animals; Rats; Gene Expression Regulation; Liver/enzymology; Antibodies/immunology; Cricetinae; Enzyme Induction/drug effects; Mesocricetus; Aflatoxin B1; Liver/drug effects/*enzymology; Methylcholanthrene/*pharmacology; Aflatoxins/immunology/*metabolism; Cytochrome P-450 Enzyme System/biosynthesis/isolation & purification/*metabolism; Mutagenicity Tests; Salmonella typhimurium/enzymology/genetics; Subcellular Fractions/drug effects/enzymology; Inbred Strains; Enzymologic; Microsomes

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