Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.

Title

Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.

Creator

Pung Yuh Fen; Rocic Petra; Murphy Michael P; Smith Robin A J; Hafemeister Jennifer; Ohanyan Vahagn; Guarini Giacinta; Yin Liya; Chilian William M

Publisher

Arteriosclerosis, thrombosis, and vascular biology

Date

2012
2012-02

Description

OBJECTIVE: We have previously found abrogated ischemia-induced coronary collateral growth in Zucker obese fatty (ZOF) rats compared with Zucker lean (ZLN) rats. Because ZOF rats have structural abnormalities in their mitochondria suggesting dysfunction and also show increased production of O(2), we hypothesized that mitochondrial dysfunction caused by oxidative stress impairs coronary collateral growth in ZOF. METHODS AND RESULTS: Increased levels of reactive oxygen species were observed in aortic endothelium and smooth muscle cells in ZOF rats compared with ZLN rats. Reactive oxygen species levels were decreased by the mitochondria-targeted antioxidants MitoQuinone (MQ) and MitoTempol (MT) as assessed by MitoSox Red and dihydroethidine staining. Lipid peroxides (a marker of oxidized lipids) were increased in ZOF by approximately 47% compared with ZLN rats. The elevation in oxidative stress was accompanied by increased antioxidant enzymes, except glutathione peroxidase-1, and by increased uncoupling protein-2 in ZOF versus ZLN rats. In addition, elevated respiration rates were also observed in the obese compared with lean rats. Administration of MQ significantly normalized the metabolic profiles and reduced lipid peroxides in ZOF rats to the same level observed in lean rats. The protective effect of MQ also suppressed the induction of uncoupling protein-2 in the obese rats. Resolution of mitochondrial oxidative stress by MQ or MT restored coronary collateral growth to the same magnitude observed in ZLN rats in response to repetitive ischemia. CONCLUSIONS: We conclude that mitochondrial oxidative stress and dysfunction play a key role in disrupting coronary collateral growth in obesity and the metabolic syndrome, and elimination of the mitochondrial oxidative stress with MQ or MT rescues collateral growth.

Subject

Animal; Animals; Antioxidants/*pharmacology; Collateral Circulation/*drug effects/physiology; Coronary Vessels/drug effects/*growth & development; Disease Models; Heart/*drug effects/physiology; Lipid Peroxidation/drug effects/physiology; Lipid Peroxides/metabolism; Male; Metabolic Syndrome/*metabolism/physiopathology; Mitochondria; Mitochondrial Proteins/metabolism; Obesity/*metabolism/physiopathology; Organophosphorus Compounds/pharmacology; Oxidative Stress/*drug effects/physiology; Piperidines/pharmacology; Rats; Reactive Oxygen Species/metabolism; Ubiquinone/pharmacology; Zucker

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

325–334

Issue

2

Volume

32

Citation

Pung Yuh Fen; Rocic Petra; Murphy Michael P; Smith Robin A J; Hafemeister Jennifer; Ohanyan Vahagn; Guarini Giacinta; Yin Liya; Chilian William M, “Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.,” NEOMED Bibliography Database, accessed November 28, 2021, https://neomed.omeka.net/items/show/4739.

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