Metabolic labeling with heavy water followed by LC-MS is a high throughput approach to study proteostasis in vivo. Advances in mass spectrometry and sample processing have allowed consistent detection of thousands of proteins at multiple time points.…
Metal homeostasis is increasingly being evaluated as a therapeutic target in stroke and neurodegenerative diseases. Metal dysregulation has been shown to lead to protein aggregation, plaque formation and neuronal death. In 2007, we first reported…
The interaction of chloroform with bilayers of dimyristoylphosphatidylcholine (DMPC) has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR). Orientational order has been measured as a function of temperature at many sites in…
Mixed background SHP(-/-) mice are resistant to diet-induced obesity due to increased energy expenditure caused by enhanced PGC-1 alpha expression in brown adipocytes. However, congenic SHP(-/-) mice on the C57BL/6 background showed normal expression…
Glutamate and aspartate are the primary neurotransmitters of projections from motor and premotor cortices to the striatum. Release of glutamate may be modulated by dopamine receptors located on corticostriatal terminals. The present study used…
The development of advanced materials that facilitate hyaline cartilage formation and regeneration in aging populations is imperative. Critical to the success of this endeavor is the optimization of ECM production from clinically relevant cells.…
The effect of 1-palmitoyl lysophosphatidylcholine (PLPC) on the phase behaviour of 1,2-dipalmitoyl phosphatidylethanolamine (DPPE) in excess water (34 wt%) has been examined by differential scanning calorimetry, scanning dilatometry and isothermal…
