A fluorinated detergent, CF(3)(CF(2))(5)C(2)H(4)-O-maltose, was reconstituted into a lipid bilayer model membrane system to demonstrate the feasibility of determining solvent accessibility and membrane immersion depth of each fluorinated group by…
The interaction of eight n-alkanols with bilayers of dimyristoylphosphatidylcholine (DMPC) has been studied by deuterium nuclear magnetic resonance (2H-NMR). At comparable temperatures and concentrations of solute in the bilayer, order parameters…
Phase properties of aqueous dispersions of model bile mixtures containing cholesterol, lecithin and bile salts and model mixed lipid digestive mixtures containing cholesterol, fatty acid, monoglyceride, lecithin and bile salts have been characterized…
H-2 nuclear magnetic resonance (NMR) spectra were obtained at 30.87 MHz for 8% (w/v) aqueous dispersions of mixtures of bile salts (MBS), mixed intestinal lipids (MIL; myristic acid, monomyristoylglycerol dimyristoylphos-phatidylcholine = 5:1:1), and…
2H nuclear magnetic resonance (NMR) spectra were obtained at 30.87 MHz for 8% (w/v) aqueous dispersions of mixtures of bile salts (MBS), mixed intestinal lipids (MIL; myristic acid, monomyristoylglycerol, dimyristoylphosphatidylcholine = 5:1:1), and…
The effect of incorporation of 1-palmitoyl-sn-glycero-3-phosphocholine (PLPC) on the structure of the P-beta' ripple mesophase in aqueous dispersions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) has been studied by differential scanning…
The pulsed field gradient NMR method for measuring self-diffusion has been used for a direct determination of the lateral diffusion coefficient of cholesterol, fluorine labeled at the 6-position, for an oriented lamellar liquid-crystalline phase of…
By isotopical labeling lipid lateral diffusion coefficients for each of the membrane constituents, including cholesterol, have been measured by H-1, H-2, and F-19 pulsed field gradient NMR spectroscopy in macroscopically oriented lipid bilayers. This…
We used an automated Langmuir-Pockels surface balance to characterize the air-water interfacial properties of cholesterol (CH) and its derivatives with hydrophilic OH and F substitutions at isologous sites on the sterol body or side chain. We studied…
