Browse Items · NEOMED Bibliography Database

Regulation of CYP4F2 leukotriene B4 omega-hydroxylase by retinoic acids in HepG2 cells.

The human CYP4F2 gene encodes a LTB4 omega-hydroxylase P450 prominently expressed in liver and kidney that functions to metabolize and inactivate the pro-inflammatory eicosanoids, LTB4 and arachidonic acid. HepG2 cells transfected with CYP4F2 -506/-6…

Promoter activity and regulation of the CYP4F2 leukotriene B(4) omega-hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells.

The human liver CYP4F2 gene (Accession No. AF221943) encodes a leukotriene B(4) omega-hydroxylase that metabolizes leukotriene B(4) (LTB(4)) to a less potent proinflammatory eicosanoid, 20-OH-LTB(4). We sequenced a 6.7-kb genomic fragment of the…

Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.

Sterol 12alpha-hydroxylase catalyzes the synthesis of cholic acid and controls the ratio of cholic acid over chenodeoxycholic acid in the bile. Transcription of CYP8B1 is inhibited by bile acids, cholesterol, and insulin. To study the mechanism of…

The complete nucleotide sequence of human liver cytochrome b5 mRNA.

We have isolated and sequenced a cDNA clone corresponding to human liver cytochrome b5 mRNA. The 760 base pair (bp) sequence contains the complete coding and 3' non-translated regions plus 52 bp of 5' non-translated sequence. The derived amino acid…

Tags: 1988, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, DNA/genetics, Humans, Liver/*metabolism, Messenger/*genetics, Molecular, Molecular Sequence Data, RNA, Steggles A W, Yoo M

The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.

We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A)…

Tags: 1989, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, DNA/genetics, Humans, Liver/*physiology, Molecular, Molecular Sequence Data, Post-Transcriptional, Pseudogenes, RNA Processing, Steggles A W, Yoo M

Physical map of the tolC-htrP region of the Escherichia coli chromosome.

Tags: *Chromosome Mapping, *Chromosomes, *Escherichia coli Proteins, *Intramolecular Transferases, 1992, Bacterial, Bacterial Outer Membrane Proteins/genetics, Bacterial Proteins/*genetics, Deoxyribonucleases, Depew R E, Escherichia coli/*genetics, Heat-Shock Proteins, Journal of bacteriology, Membrane Transport Proteins, Molecular Sequence Data, Restriction Mapping, Type II Site-Specific/metabolism, Yang T P

Nucleotide sequence of a region duplicated in Escherichia coli toc mutants.

We have sequenced about 5 kb of the Escherichia coli chromosome downstream from the tolC gene, looking for a topoisomerase gene. This region does not contain a topoisomerase gene.

Tags: *Genes, *Multigene Family, 1992, Bacterial, Base Sequence, Biochimica et biophysica acta, Depew R E, DNA Topoisomerases, Escherichia coli/*genetics, Molecular Sequence Data, Type I/genetics, Yang T P

Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.

Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA…

Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.

Oxysterol 7 alpha-hydroxylase catalyzes hydroxylation of oxysterols and neurosterols and plays a role in the alternative bile acid synthesis pathway. This gene is widely expressed in many organs and peripheral tissues and may protect tissues from the…

Binding of sequence-specific proteins to the 3'-untranslated region of vasoactive intestinal peptide mRNA.

Multimeric AUUUA elements in an AU-rich 3'-untranslated region (3'-UTR) have been shown to confer message instability to numerous ephemeral transcripts through the formation of RNA-protein complexes. We show here that the 3'-UTR of VIP mRNA, which…

Analysis of the thymidine kinase of a herpes simplex virus type 1 isolate that exhibits resistance to (E)-5-(2-bromovinyl)-2'-deoxyuridine.

The mechanism responsible for the decreased sensitivity of a clinical herpes simplex virus type 1 (HSV-1) isolate, HSV-145, to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was examined. Measurements of 50% inhibitory doses of several drugs…

Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.

A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol

Structure and nucleotide sequences of the human cholesterol 7 alpha-hydroxylase gene (CYP7).

The human cholesterol 7 alpha-hydroxylase gene (CYP7) spans about 11 kb of the genome and contains six exons and five introns. Nucleotide sequences of a 5'-upstream region to exon III (5535 bp), a 5'-upstream EcoRI fragment (2575 bp), and an EcoRI…

Tags: 1994, Amino Acid Sequence, Base Sequence, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/chemistry/*genetics, Department of Integrative Medical Sciences, DNA, Genomics, Humans, Molecular Sequence Data, NEOMED College of Medicine, Restriction Mapping, Wang D P

The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.

In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue. When the meat is cooked, a disagreeable odor/flavor is produced, a phenomenon known as "boar taint." All…

Tags: 1997, Amino Acid Sequence, Animals, Base Sequence, Biochemical and biophysical research communications, Blotting, Complementary/blood/*chemistry/*isolation & purification, Cytochromes b5/blood/*chemistry/genetics/*isolation & purification, DNA, Male, Membrane Proteins/*chemistry/genetics/*isolation & purification, Molecular Sequence Data, Solubility, Southern, Steggles A W, Swine, Testis/enzymology, VanDerMark P K

Expression of isotocin-neurophysin mRNA in developing zebrafish.

Neurohypophysial peptides are important regulators of homeostasis, reproduction and behavior. We have sequenced a zebrafish cDNA representing isotocin-neurophysin (IT-NP) mRNA. The developmental expression pattern of zebrafish IT-NP mRNA was…

Tags: 2003, Amino Acid Sequence, Animals, Gene expression patterns : GEP, Glasgow Eric, Messenger/*metabolism, Molecular Sequence Data, Neurophysins/biosynthesis/*genetics, Oxytocin/*analogs & derivatives/biosynthesis/*genetics, Phylogeny, RNA, Unger Jennifer L, Zebrafish/*embryology

Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.

Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This…

Immunization with a LEAPS heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1.

A ligand epitope antigen presentation system (LEAPS) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from beta-2-microglobulin (35-50) elicited protection from…

The role of the N-terminal and mid-region residues of substance P in regulating functional selectivity at the tachykinin NK1 receptor.

Previous studies have shown that tachykinin peptide ligands of the tachykinin NK1 receptor exhibit functional selectivity with respect to signal activation and desensitization. The differences are most dramatic between the naturally occurring…

Transcriptional regulation of human oxysterol 7alpha-hydroxylase by sterol response element binding protein.

Oxysterol 7alpha-hydroxylase (CYP7B1) metabolizes oxysterols, potent regulators of lipid homeostasis. Very little is known about transcriptional regulation of human CYP7B1. The present results indicate that sterol response element binding protein…

An N-terminal arginine-rich cluster and a proline-alanine-threonine repeat region determine the cellular localization of the herpes simplex virus type 1 ICP34.5 protein and its ligand, protein phosphatase 1.

The ICP34.5 protein facilitates herpes simplex virus replication by binding and activating protein phosphatase 1 (PP1) by means of a very conserved C-terminal

Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.

Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of…

The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.

We have recently cloned a full-length cDNA encoding the rat hepatic cholesterol 7 alpha-hydroxylase cytochrome P450 (P450c7) (Li, Y. C., Wang, D. P., and Chiang, J. Y. L. (1990) J. Biol. Chem. 265, 12012-12019), which catalyzes the rate-limiting…

The isolation and characterization of the human cytochrome b5 gene.

From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid…

PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine.

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and

Tags: *Lipid Metabolism, 2007, ATP Binding Cassette Transporter, ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters/genetics, Base Sequence, Cell Line, Chen Wenling, Chiang John Y L, Cholestanetriol 26-Monooxygenase/*metabolism, Cholesterol, Cholesterol/*metabolism, Department of Integrative Medical Sciences, Fluorinated, Genes, Genetic/drug effects, Genetic/genetics, HDL/metabolism, Hepatocytes/drug effects/enzymology/metabolism, Humans, Hydrocarbons, Hydroxycholesterols/metabolism/pharmacology, Intestinal Mucosa/metabolism, Intestines/cytology/drug effects/enzymology, Journal of lipid research, Li Tiangang, Member 1, Messenger/genetics/metabolism, Molecular Sequence Data, NEOMED College of Medicine, Pregnane X Receptor, Promoter Regions, Receptors, Reporter, Response Elements/genetics, Rifampin/pharmacology, RNA, Steroid/*metabolism, Subfamily G, Sulfonamides/pharmacology, Transcription, Up-Regulation/drug effects

IL-1beta-induced cortisol stimulates lung fluid absorption in fetal guinea pigs via SGK-mediated Nedd4-2 inhibition.

We tested the hypothesis that interleukin (IL)-1beta-induced cortisol synthesis stimulates distal lung fluid absorption in fetal guinea pigs via induction of serum- and glucocorticoid-regulated kinase (SGK) and inhibition of neural precursor cell…

Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.

We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two…

Generation and characterization of ATP analog-specific protein kinase Cdelta.

To better study the role of PKCdelta in normal function and disease, we developed an ATP analog-specific (AS) PKCdelta that is sensitive to specific kinase inhibitors and can be used to identify PKCdelta substrates. AS PKCdelta showed nearly 200…

Polymorphisms of human cholesterol 7 alpha-hydroxylase.

Human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs were isolated from a human liver cDNA library. A full-length cDNA has 2901 nucleotides which encode a typical P450 polypeptide of 504 amino acid residues. Two different sequences of codon 100,…

Tags: 1992, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, Cloning, Department of Integrative Medical Sciences, DNA/genetics, Genetic, Humans, Karam W G, Molecular, Molecular Sequence Data, NEOMED College of Medicine, Oligodeoxyribonucleotides/chemistry, Polymerase Chain Reaction, Polymorphism, Restriction Mapping

Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Cholesterol 7 alpha-hydroxylase (P450c7) is the first and rate-limiting enzyme in bile acid biosynthesis and is the product of a cytochrome P450 gene, CYP7. We have previously reported the cloning of a full-length human cholesterol 7…

Protein thiyl radical mediates S-glutathionylation of complex I.

Complex I is a critical site of O(2)(*-) production and the major host of reactive protein thiols in mitochondria. In response to oxidative stress, complex I protein thiols at the 51- and 75-kDa subunits are reversibly

Tags: *Oxidative Stress, 2012, Amino Acid Motifs, Amino Acid Sequence, Animals, Binding Sites, Cattle, Cell Line, Chen Chwen-Lih, Chen Jingfeng, Chen Yeong-Renn, Cyclic N-Oxides/chemistry/pharmacology, Cysteine/chemistry/*metabolism, Department of Integrative Medical Sciences, Electron Transport Complex I/chemistry/*metabolism, Free radical biology & medicine, Free Radical Scavengers/chemistry/pharmacology, Free Radicals/chemistry/*metabolism, Glutathione/chemistry/*metabolism, Green Kari B, Heart/enzymology/metabolism, Kang Patrick T, Mice, Mitochondria, Models, Molecular, Molecular Sequence Data, Muscle Cells/drug effects/metabolism, NEOMED College of Medicine, Onium Compounds/pharmacology, Peptide Fragments/chemistry, Peptide Mapping, Protein, Rats, Rotenone/pharmacology, Structural Homology, Superoxides/metabolism, Zhang Liwen

Specificity of metabotropic glutamate receptor 2 coupling to G proteins.

Metabotropic glutamate receptor 2 (mGluR2) is a class 3 G protein-coupled receptor and an important mediator of synaptic activity in the central nervous system. Previous work demonstrated that mGluR2 couples to pertussis toxin (PTX)-sensitive G…

A L.E.A.P.S. heteroconjugate vaccine containing a T cell epitope from HSV-1 glycoprotein D elicits Th1 responses and protection.

The L.E.A.P.S. heteroconjugate vaccine antigen (JgD), composed of a T cell epitope from glycoprotein D (gD(8-23)) of herpes simplex virus (HSV) linked with a peptide sequence from beta-2-microglobulin (aa38-50), elicited protection against lethal…

APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus.

Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial…

Tags: 2005, 3' Untranslated Regions/genetics, Amino Acid, Animals, Antisense/*genetics/isolation & purification/*metabolism, Brain research. Molecular brain research, Conserved Sequence/genetics, DNA-Binding Proteins/genetics/*metabolism, Gainer Harold, Gene Expression Regulation/*genetics, Genetic/genetics, Genomic Imprinting/genetics, Glasgow Eric, Hypothalamo-Hypophyseal System/cytology/metabolism, Kruppel-Like Transcription Factors, Male, Messenger/genetics/metabolism, Molecular Sequence Data, Mutsuga Noriko, Neurons/*metabolism, Nucleic Acid, Protein Kinases/genetics/*metabolism, Rats, RNA, Ryu Seung-Lim, Sequence Homology, Sprague-Dawley, Supraoptic Nucleus/cytology/*metabolism, Transcription, Transcription Factors/genetics/*metabolism, Vasopressins/*metabolism, Water-Electrolyte Balance/genetics, Yamashita Mitsuo, Zhang Bing-Jun

The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.

Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to…

Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.

RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products…

Tags: 1992, Amino Acid Sequence, Animals, Base Sequence, Cytochromes b5/chemistry/*genetics, Giordano S J, Leukocytes/*chemistry, Liver/chemistry, Messenger/*chemistry, Molecular Sequence Data, Nucleic Acid, Nucleic Acid Hybridization, Polymerase Chain Reaction, Rabbits, Reticulocytes/*chemistry, RNA, SAAS bulletin, biochemistry and biotechnology, Sequence Homology, Steggles A W

Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.

Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed.…

The human liver and reticulocyte cytochrome b5 mRNAs are products from a single gene.

Using a combination of standard cDNA library screening techniques and the Polymerase Chain Reaction (PCR) we have isolated and sequenced DNA fragments corresponding to the human reticulocyte cytochrome b5 mRNA. The reticulocyte specific sequence…

Tags: *Genes, 1991, Amino Acid Sequence, Base Sequence, Biochemical and biophysical research communications, Cytochromes b5/blood/*genetics, Gene Library, Genetic, Giordano S J, Humans, Liver/*metabolism, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Reticulocytes/*metabolism, RNA/blood/genetics/isolation & purification, Steggles A W

Tags: 1988, Amino Acid Sequence, Animals, Base Sequence, Chromosome Deletion, Cloning, Cytochrome b Group/*genetics, Cytochromes b5, Dariush N, Fisher C W, Genes, Liver/*metabolism, Messenger/*genetics, Molecular, Molecular Sequence Data, Protein sequences & data analysis, Rabbits, RNA, Steggles A W

Browse Items (53 total)