Browse Items · NEOMED Bibliography Database

Histone deacetylase inhibitor vorinostat (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1beta-induced IL-6 expression in human OA chondrocytes.

AIM OF THE STUDY: High levels of IL-6 are believed to contribute to osteoarthritis (OA) pathogenesis. The expression of IL-6 is regulated post-transcriptionally by the miR-9-MCPIP-1 axis in chondrocytes. Vorinostat (SAHA) inhibits the IL-6 expression…

Monoamine-activated alpha 2-macroglobulin inhibits choline acetyltransferase of embryonic basal forebrain neurons and reversal of the inhibition by NGF and BDNF but not NT-3.

Monoamine-activated alpha 2-macroglobulin (alpha 2M) has recently been shown to inhibit the growth and survival of cholinergic neurons of the basal forebrain (Liebl and Koo: J Neurosci Res 35:170-182, 1993). The mechanism of this inhibitory effect is…

Tags: 1994, alpha-Macroglobulins/antagonists & inhibitors/*pharmacology, Animals, Biogenic Monoamines/*pharmacology, Brain-Derived Neurotrophic Factor, Cells, Choline O-Acetyltransferase/*antagonists & inhibitors, Cultured, Humans, Journal of neuroscience research, Koo P H, Liebl D J, Nerve Growth Factors/*pharmacology, Nerve Tissue Proteins/pharmacology, Neurons/drug effects/*metabolism, Neurotrophin 3, Prosencephalon/*cytology, Rats

Serotonin-activated alpha 2-macroglobulin inhibits neurite outgrowth and survival of embryonic sensory and cerebral cortical neurons.

Methylamine-modified alpha-2-macroglobulin (MA-alpha 2M) has been recently shown to inhibit the biological activity of beta-nerve growth factor (NGF) in promoting neurite outgrowth by embryonic dorsal root ganglia in culture (Koo PH, Liebl DJ, J…

The isolation and characterization of the human cytochrome b5 gene.

From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid…

Bile acids and cytokines inhibit the human cholesterol 7 alpha-hydroxylase gene via the JNK/c-jun pathway in human liver cells.

Cholesterol 7 alpha-hydroxylase (CYP7A1) of the bile acid biosynthesis pathway is suppressed by bile acids and inflammatory cytokines. Bile acids are known to induce inflammatory cytokines to activate the mitogen-activated protein kinase/c-Jun…

Mechanism of rifampicin and pregnane X receptor inhibition of human cholesterol 7 alpha-hydroxylase gene transcription.

Bile acids, steroids, and drugs activate steroid and xenobiotic receptor pregnane X receptor (PXR; NR1I2), which induces human cytochrome P4503A4 (CYP3A4) in drug metabolism and cholesterol 7 alpha-hydroxylase (CYP7A1) in bile acid synthesis in the…

A novel role of transforming growth factor beta1 in transcriptional repression of human cholesterol 7alpha-hydroxylase gene.

BACKGROUND & AIMS: Inhibition of cholesterol 7alpha-hydroxylase (CYP7A1) by bile acids and inflammatory cytokines provides an important mechanism to protect hepatocytes from bile acid toxicity during cholestasis. Transforming growth factor beta1…

Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes.

Bile acids play important roles in the regulation of lipid, glucose, and energy homeostasis. Recent studies suggest that glucose regulates gene transcription in the liver. The aim of this study was to investigate the potential role of glucose in…

RNA interference for CFTR attenuates lung fluid absorption at birth in rats.

BACKGROUND: Small interfering RNA (siRNA) against alphaENaC (alpha-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. METHODS:…

Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

When Mg2+ or ethidium bromide (EB) were combined with poly r(A-U) at a ligand/ribonucleotide ratio of 1/4, the antiviral activity of the Mg2+ and EB increased 136-fold and 154-fold. Eriochrome Blue SE was employed to visualize the subcellular…

Tags: 1992, Antiviral Agents/*pharmacology, Cell biology international reports, Cells, Chromatin/drug effects, Cultured, Ethidium/*pharmacology, Gilloteaux J, Humans, Jamison J M, Krabill K, Magnesium/metabolism/*pharmacology, Nucleolus Organizer Region/*drug effects, Poly A-U/*pharmacology, Summers J L, Vesicular stomatitis Indiana virus/drug effects

Subcellular localization and antiviral activity of carminic acid/poly r(A-U) combinations.

Carminic acid (CAR) enhances the antiviral activity of poly r(A-U) twelve-fold without increasing interferon induction, inactivating the vesicular stomatitis virus or inducing host cell cytotoxicity. Phase contrast photomicrographs of human foreskin…

Interaction of nerve growth factor with murine alpha-macroglobulin.

The murine nerve growth factor, when injected i.v. or, combined in vitro with plasma, was found largely associated with the mouse alpha-macroglobulin (a homologue of human alpha 2-macroglobulin). The nerve growth factor-alpha-macroglobulin complex…

Tags: 1989, alpha-Macroglobulins/*metabolism, Animals, Cells, Chick Embryo, Cultured, Ganglia, Journal of neuroscience research, Koo P H, Methylamines/pharmacology, Mice, Nerve Growth Factors/*metabolism, Spinal/*cytology/drug effects, Stach R W, Trypsin/pharmacology, Zinc/pharmacology

Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

alpha 2-Macroglobulin (alpha 2M) is a rather ubiquitous protein in extracellular spaces of mammals. It is an inhibitor of endopeptidases, can be modified by aliphatic amines, and combines with a number of hormones/cytokines such as beta-nerve growth…

Inhibition of the G2/M transition of the cell cycle by methyl-2,5-dihydroxycinnamate in human lymphoid cells.

Immortalized human lymphoid cells treated with Methyl-2,5-dihydroxycinnamate (MDHC), a stable analog of erbstatin, inhibited the G2/M transition of the cell cycle. The MDHC inhibition of the cell cycle was observed at concentrations well below the…

Tags: 1996, Biochemical and biophysical research communications, Cell Line, Cinnamates/*pharmacology, Cultured, G2 Phase/*drug effects, Humans, Jamison J M, Koch J A, Lymphocytes/cytology/*drug effects, Mitosis/*drug effects, Monocytes/cytology/drug effects, Rosenthal K S, Summers J L, Transformed, Tumor Cells

Age-related expression patterns of Bag-1 and Bcl-2 in growth plate and articular chondrocytes.

Aging cartilage displays increased chondrocyte apoptosis and decreased responsiveness of chondrocytes to growth factors. The molecular mechanisms responsible for these changes have not been identified. Bag-1 is a Bcl-2-binding protein that promotes…

A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1beta-induced activation of AP-1 in human OA chondrocytes.

Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we…

Nrf2/ARE pathway attenuates oxidative and apoptotic response in human osteoarthritis chondrocytes by activating ERK1/2/ELK1-P70S6K-P90RSK signaling axis.

Nrf2, a redox regulated transcription factor, has recently been shown to play a role in cartilage integrity but the mechanism remains largely unknown. Osteoarthritis (OA) is a multifactorial disease in which focal degradation of cartilage occurs.…

Wogonin, a natural flavonoid, intercalates with genomic DNA and exhibits protective effects in IL-1beta stimulated osteoarthritis chondrocytes.

Wogonin has recently been shown to possess anti-inflammatory and chondroprotective properties and is of considerable interest due to its broad pharmacological activities. The present study highlights that Wogonin binds DNA and exerts…

Glycation Reduces the Stability of ApoAI and Increases HDL Dysfunction in Diet-Controlled Type 2 Diabetes.

Context: Hyperglycemia plays a key role in the pathogenesis of cardiovascular complications of diabetes. Type 2 diabetes mellitus (T2DM) is associated with high-density lipoprotein (HDL) dysfunction and increased degradation of apolipoprotein I…

Tags: 2018, Adult, Aged, Animal Studies, Animals, Apolipoprotein A-I/blood/*metabolism, Apolipoproteins – Blood, Apolipoproteins – Metabolism, Bena James, Biochemical Phenomena, Case Control Studies, Case-Control Studies, Cells, Comparative Studies, Cultured, Department of Pharmaceutical Sciences, Diabetes Mellitus, Diet, Dyslipidemias/complications/diet therapy/*metabolism, Evaluation Research, Female, Funding Source, Glycosylation, Golizeh Makan, HDL – Metabolism, HDL/*metabolism, Human, Humans, Hyperglycemia – Complications, Hyperglycemia – Diet Therapy, Hyperglycemia – Metabolism, Hyperglycemia/complications/diet therapy/*metabolism, Hyperlipidemia – Complications, Hyperlipidemia – Diet Therapy, Hyperlipidemia – Metabolism, Ilchenko Serguei, Kashyap Sangeeta R, Kasumov Takhar, Lee Kwangwon, Lipoproteins, Male, Mice, Middle Age, Middle Aged, Multicenter Studies, NEOMED College of Pharmacy, Osme Abdullah, Previs Stephen F, Protein Stability, Smith Jonathan D, The Journal of clinical endocrinology and metabolism, Type 2 – Complications, Type 2 – Diet Therapy, Type 2 – Metabolism, Type 2/complications/*diet therapy/metabolism, Validation Studies, Wang Shuhui

Cell cycle arrest and autoschizis in a human bladder carcinoma cell line following Vitamin C and Vitamin K3 treatment.

Exponentially growing cultures of human bladder tumor cells (T24) were treated with Vitamin C (VC) alone, Vitamin K(3) (VK(3)) alone, or with a VC:VK(3) combination for 1, 2, or 4hr. Flow cytometry of T24 cells exposed to the vitamins for 1h revealed…

Alignment of inducible vascular progenitor cells on a micro-bundle scaffold improves cardiac repair following myocardial infarction.

Ischemic heart disease is still the leading cause of death even with the advancement of pharmaceutical therapies and surgical procedures. Early vascularization in the ischemic heart is critical for a better outcome. Although stem cell therapy has…

Tags: *Cardiovascular regeneration, *Ischemic heart diseases, *Micro-bundle scaffold, *Myocardial infarction, *Neovascularization, *Stem cells, *Tissue Scaffolds, *Vascular progenitor cells, 2017, Animal, Animals, Basic research in cardiology, Cell Differentiation, Cell Proliferation, Cell Survival, Cells, Chilian William M, Coculture Techniques, Cultured, Cumpston Devan, Department of Integrative Medical Sciences, Disease Models, Dong Feng, Endothelial Progenitor Cells/metabolism/*transplantation, Enrick Molly, Fibroblast Growth Factor 2/metabolism, Hakobyan Tatev, Jamaiyar Anurag, Janota Danielle, Kolz Christopher L, Lactic Acid/*chemistry, Muscle, Myocardial Infarction/metabolism/pathology/physiopathology/*surgery, Myocardium/metabolism/*pathology, Myocytes, NEOMED College of Medicine, Newby Bi-Min Zhang, Ohanyan Vahagn, Paracrine Communication, Phenotype, Physiologic, Polyglycolic Acid/*chemistry, Polylactic Acid-Polyglycolic Acid Copolymer, Rats, Signal Transduction, Smooth, Smooth Muscle/metabolism/*transplantation, Song Hokyung, Sprague-Dawley, Stevanov Kelly, Time Factors, Tissue Engineering/*methods, Vascular Endothelial Growth Factor A/metabolism, Vascular/metabolism/*transplantation, Ventricular Remodeling, Wan Weiguo, Yin Liya

Experimental use of fibrin glue to induce site-directed osteogenesis from cultured periosteal cells.

The purpose of this study was to determine whether a combination of fibrin glue and cultured periosteal cells will result in new bone formation at heterotopic sites in nude mice. Growing cells and developing matrices surrounding periosteal explants…

Inhibition of dopamine and choline acetyltransferase concentrations in rat CNS neurons by rat alpha 1- and alpha 2-macroglobulins.

Previous studies have implicated human alpha-2-macroglobulin (alpha2M) as a potential regulator of neuronal development and function. Rat alpha-1-macroglobulin (alpha1M) and acute-phase alpha-2-macroglobulin (alpha2M) are murine homologues of human…

Type VI collagen increases cell survival and prevents anti-beta 1 integrin-mediated apoptosis.

Cell-matrix interactions are important in the development of the avian cornea. Type VI collagen is present within the periocular mesenchyme prior to the migration of cells into the corneal stroma and is abundant in the mature stroma. Whether the…

The effect of various insufflation gases on tumor implantation in an animal model.

OBJECTIVE: The expanded use of laparoscopy has led to reports of tumor dissemination and spread to laparoscopic port sites. We previously showed that carbon dioxide insufflation produced tumor dissemination compared with laparotomy. It is unknown…

Mechanism of vitamin D receptor inhibition of cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes.

Lithocholic acid (LCA) is a potent endogenous vitamin D receptor (VDR) ligand. In cholestasis, LCA levels increase in the liver and intestine. The objective of this study is to test the hypothesis that VDR plays a role in inhibiting cholesterol…

Prostate apoptosis response-4 enhances secretion of amyloid beta peptide 1-42 in human neuroblastoma IMR-32 cells by a caspase-dependent pathway.

Prostate apoptosis response-4 (Par-4) is a leucine zipper protein that promotes neuronal cell death in Alzheimer's disease (AD). Neuronal degeneration in AD may result from extracellular accumulation of amyloid beta peptide (Abeta) 1-42. To examine…

Mechanosensitive transient receptor potential vanilloid 4 regulates Dermatophagoides farinae-induced airway remodeling via 2 distinct pathways modulating matrix synthesis and degradation.

Contributions of mechanical signals to airway remodeling during asthma are poorly understood. Transient receptor potential vanilloid 4 (TRPV4), a mechanosensitive ion channel, has been implicated in cardiac and pulmonary fibrosis; however, its role…

Tags: *Airway Remodeling, *fibronectin, *myofibroblasts, *PAI-1, *PI3K, *TGF-beta1, 2017, Adult, Al-Azzam Nosayba, Animals, Asthma/etiology/genetics/*metabolism/pathology, Cells, Cultured, Department of Integrative Medical Sciences, Dermatophagoides farinae/immunology, Duah Ernest, Extracellular Matrix/*metabolism/pathology, FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Fibroblasts/metabolism, Fibronectins/*metabolism, Gombedza Farai, Hexter Madison, Humans, Inbred C57BL, Kondeti Vinay, Koppes Stephanie, Male, Mice, NEOMED College of Medicine, Paruchuri Sailaja, Patil Prachi, Phillips Daniel, Phosphatidylinositol 3-Kinases/metabolism, Plasminogen Activator Inhibitor 1/metabolism, Thodeti Charles K, Transforming Growth Factor beta/metabolism, TRPV Cation Channels/genetics/*metabolism

Scanning electron microscopy and transmission electron microscopy aspects of synergistic antitumor activity of vitamin C - vitamin K3 combinations against human prostatic carcinoma cells.

A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor…

Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro.

Atherosclerosis, a major macrovascular complication associated with diabetes, poses a tremendous burden on national health care expenditure. Despite extensive efforts, cost-effective remedies are unknown. Therapies for atherosclerosis are challenged…

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

Trivalent chromium (Cr(3+)) is a mineral nutrient reported to have beneficial effects in glycemic and cardiovascular health. In vitro and in vivo studies suggest that Cr(3+) supplementation reduces the atherogenic potential and lowers the risk of…

Tags: 2015, American journal of physiology. Cell physiology, Aorta/drug effects/metabolism, Cell Proliferation/*drug effects/genetics, Cells, Chavez Ronaldo J, Chromium/*pharmacology, Cultured, Department of Integrative Medical Sciences, Fructosephosphates/metabolism, Ganguly Rituparna, Genetic/drug effects/genetics, Glucose/*metabolism, Glutamine/genetics, Glycosylation/drug effects, Hexosamines/metabolism, Humans, Hyperglycemia/metabolism, Muscle, Myocytes, N-Acetylglucosaminyltransferases/genetics, NEOMED College of Medicine, O-glycosylation, Promoter Regions, Raman Priya, reactive oxygen species, Reactive Oxygen Species/metabolism, Sahu Soumyadip, Signal Transduction/*drug effects/genetics, Smooth, Smooth Muscle/*drug effects/metabolism, Thrombospondin 1/*antagonists & inhibitors/genetics, thrombospondin-1, Transcription, trivalent chromium, vascular smooth muscle cells, Vascular/*drug effects/metabolism

Basic transcription element binding protein (BTEB) transactivates the cholesterol 7 alpha-hydroxylase gene (CYP7A).

Cholesterol 7 alpha-hydroxylase catalyzes the first and rate-limiting step in the conversion of cholesterol to bile acids in the liver. Previously, we have identified two bile acid response elements located in nt -74 to -54 (BARE-I) and -148 to -118…

Pertubation of beta1 integrin function using anti-sense or function-blocking antibodies on corneal cells grown on fibronectin and tenascin.

During corneal development, neural crest derivatives from the periocular mesenchyme migrate into the cornea and differentiate into corneal fibroblasts. During this time, these cells interact with a variety of extracellular matrices for proper…

Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).

The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion…

Transcriptional activation of the cholesterol 7alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors.

The gene encoding cholesterol 7alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the…

Tags: *Receptors, *Transcriptional Activation, 1998, Animals, Base Sequence, Bile Acids and Salts/biosynthesis, Chiang J Y, Cholesterol 7-alpha-Hydroxylase/*genetics, COUP Transcription Factor II, COUP Transcription Factors, Crestani M, Cultured, Cytoplasmic and Nuclear/*physiology, Department of Integrative Medical Sciences, DNA-Binding Proteins/metabolism, DNA/metabolism, Galli G, Genes, Genetic, Hepatocyte Nuclear Factor 4, Hormones/*physiology, Journal of lipid research, Luciferases/genetics, Molecular Sequence Data, Mutagenesis, NEOMED College of Medicine, Nucleic Acid, Oligonucleotide Probes/metabolism, Phosphoproteins/metabolism, Promoter Regions, Rats, Receptors, Repetitive Sequences, Reporter, Retinoic Acid/metabolism, Retinoid X Receptors, Sadeghpour A, Site-Directed, Steroid, Stroup D, Transcription Factors/metabolism, Tumor Cells

Effects of bile acids and steroid/thyroid hormones on the expression of cholesterol 7 alpha-hydroxylase mRNA and the CYP7 gene in HepG2 cells.

The expression of cholesterol 7 alpha-hydroxylase mRNA levels in confluent HepG2 cultures was reduced by tauro- or glyco-conjugates of deoxycholate and chenodeoxycholate, but not by cholate. Ursodeoxycholates, on the other hand, stimulated the mRNA…

Inhibition of human papillomavirus type 16 gene expression by nordihydroguaiaretic acid plant lignan derivatives.

Several methylated derivatives of a plant lignan, nordihydroguaiaretic acid (NDGA) were found to be potent anti-viral agents by suppressing Sp1 regulated transcription within the sexually transmitted viruses human immunodeficiency virus (HIV) and…

Farnesoid X receptor responds to bile acids and represses cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription.

Cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription is repressed by bile acids. The goal of this study is to elucidate the mechanism of CYP7A1 transcription by bile acid-activated farnesoid X receptor (FXR) in its native promoter and cellular…

Regulation of cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRalpha).

The cholesterol 7alpha-hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. Oxysterol receptor, LXR, stimulates, whereas the bile acid receptor, FXR, inhibits CYP7A1 transcription. The…

Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4alpha (HNF4alpha).

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyses sterol side-chain oxidation of bile acid synthesis from cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27A1 gene…

Tags: *DNA-Binding Proteins, 2003, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Bile Acids and Salts/*pharmacology, Binding Sites/genetics, Cell Line, Chen Wenling, Chenodeoxycholic Acid/pharmacology, Chiang John Y L, Cholestanetriol 26-Monooxygenase, Cloning, Cultured, Cytoplasmic and Nuclear/genetics/metabolism, Department of Integrative Medical Sciences, DNA, DNA/chemistry/genetics, Dose-Response Relationship, Drug, gene, Gene Expression Regulation/drug effects, Genetic/*genetics, Hepatocyte Nuclear Factor 4, Humans, Luciferases/genetics/metabolism, Molecular, Molecular Sequence Data, Mutagenesis, Mutation, NEOMED College of Medicine, Phosphoproteins/genetics/*metabolism, Promoter Regions, Receptors, Recombinant Fusion Proteins/genetics/metabolism, Response Elements/genetics, Sequence Analysis, Site-Directed, Steroid Hydroxylases/*genetics, Transcription Factors/genetics/*metabolism, Transfection, Tumor Cells

Browse Items (87 total)