Browse Items · NEOMED Bibliography Database

A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1beta-induced activation of AP-1 in human OA chondrocytes.

Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we…

Age-related expression patterns of Bag-1 and Bcl-2 in growth plate and articular chondrocytes.

Aging cartilage displays increased chondrocyte apoptosis and decreased responsiveness of chondrocytes to growth factors. The molecular mechanisms responsible for these changes have not been identified. Bag-1 is a Bcl-2-binding protein that promotes…

Inhibition of nerve growth factor-stimulated neurite outgrowth by methylamine-modified alpha 2-macroglobulin.

alpha 2-Macroglobulin (alpha 2M) is a rather ubiquitous protein in extracellular spaces of mammals. It is an inhibitor of endopeptidases, can be modified by aliphatic amines, and combines with a number of hormones/cytokines such as beta-nerve growth…

Interaction of nerve growth factor with murine alpha-macroglobulin.

The murine nerve growth factor, when injected i.v. or, combined in vitro with plasma, was found largely associated with the mouse alpha-macroglobulin (a homologue of human alpha 2-macroglobulin). The nerve growth factor-alpha-macroglobulin complex…

Tags: 1989, alpha-Macroglobulins/*metabolism, Animals, Cells, Chick Embryo, Cultured, Ganglia, Journal of neuroscience research, Koo P H, Methylamines/pharmacology, Mice, Nerve Growth Factors/*metabolism, Spinal/*cytology/drug effects, Stach R W, Trypsin/pharmacology, Zinc/pharmacology

Subcellular localization and antiviral activity of carminic acid/poly r(A-U) combinations.

Carminic acid (CAR) enhances the antiviral activity of poly r(A-U) twelve-fold without increasing interferon induction, inactivating the vesicular stomatitis virus or inducing host cell cytotoxicity. Phase contrast photomicrographs of human foreskin…

Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

When Mg2+ or ethidium bromide (EB) were combined with poly r(A-U) at a ligand/ribonucleotide ratio of 1/4, the antiviral activity of the Mg2+ and EB increased 136-fold and 154-fold. Eriochrome Blue SE was employed to visualize the subcellular…

Tags: 1992, Antiviral Agents/*pharmacology, Cell biology international reports, Cells, Chromatin/drug effects, Cultured, Ethidium/*pharmacology, Gilloteaux J, Humans, Jamison J M, Krabill K, Magnesium/metabolism/*pharmacology, Nucleolus Organizer Region/*drug effects, Poly A-U/*pharmacology, Summers J L, Vesicular stomatitis Indiana virus/drug effects

Tissue Engineering A Model For The Human Ear: Assessment Of Size, Shape, Morphology, And Gene Expression Following Seeding Of Different Chondrocytes

This study examines the tissue engineering of a human ear model through use of bovine chondrocytes isolated from four different cartilaginous sites (nasoseptal, articular, costal, and auricular) and seeded onto biodegradable poly(l-lactic acid) and…

Impact Of Parturition On Chemokine Homing Factor Expression In The Vaginal Distention Model Of Stress Urinary Incontinence

Purpose: Human childbirth simulated by vaginal distention is known to increase the expression of chemokines and receptors involved in stem cell homing and tissue repair. We hypothesized that pregnancy and parturition in rats contributes to the…

RNA interference for CFTR attenuates lung fluid absorption at birth in rats.

Background: Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. Methods: Twenty-four…

RNA interference for CFTR attenuates lung fluid absorption at birth in rats.

BACKGROUND: Small interfering RNA (siRNA) against alphaENaC (alpha-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. METHODS:…

Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes.

Bile acids play important roles in the regulation of lipid, glucose, and energy homeostasis. Recent studies suggest that glucose regulates gene transcription in the liver. The aim of this study was to investigate the potential role of glucose in…

A novel role of transforming growth factor beta1 in transcriptional repression of human cholesterol 7alpha-hydroxylase gene.

BACKGROUND & AIMS: Inhibition of cholesterol 7alpha-hydroxylase (CYP7A1) by bile acids and inflammatory cytokines provides an important mechanism to protect hepatocytes from bile acid toxicity during cholestasis. Transforming growth factor beta1…

Bile acids and cytokines inhibit the human cholesterol 7 alpha-hydroxylase gene via the JNK/c-jun pathway in human liver cells.

Cholesterol 7 alpha-hydroxylase (CYP7A1) of the bile acid biosynthesis pathway is suppressed by bile acids and inflammatory cytokines. Bile acids are known to induce inflammatory cytokines to activate the mitogen-activated protein kinase/c-Jun…

Serotonin-activated alpha 2-macroglobulin inhibits neurite outgrowth and survival of embryonic sensory and cerebral cortical neurons.

Methylamine-modified alpha-2-macroglobulin (MA-alpha 2M) has been recently shown to inhibit the biological activity of beta-nerve growth factor (NGF) in promoting neurite outgrowth by embryonic dorsal root ganglia in culture (Koo PH, Liebl DJ, J…

Monoamine-activated alpha 2-macroglobulin inhibits choline acetyltransferase of embryonic basal forebrain neurons and reversal of the inhibition by NGF and BDNF but not NT-3.

Monoamine-activated alpha 2-macroglobulin (alpha 2M) has recently been shown to inhibit the growth and survival of cholinergic neurons of the basal forebrain (Liebl and Koo: J Neurosci Res 35:170-182, 1993). The mechanism of this inhibitory effect is…

Tags: 1994, alpha-Macroglobulins/antagonists & inhibitors/*pharmacology, Animals, Biogenic Monoamines/*pharmacology, Brain-Derived Neurotrophic Factor, Cells, Choline O-Acetyltransferase/*antagonists & inhibitors, Cultured, Humans, Journal of neuroscience research, Koo P H, Liebl D J, Nerve Growth Factors/*pharmacology, Nerve Tissue Proteins/pharmacology, Neurons/drug effects/*metabolism, Neurotrophin 3, Prosencephalon/*cytology, Rats

Stem Cell Therapies For Coronary Collateral Growth In Zucker Obese Fatty Rats: Redox Modulator Mitoneet As A Therapeutic Target

Tags: 2013, Arteriogenesis, Cardiovascular System & Cardiology, Carroll R, Cells, Chilian W, Circulation, Enrick M, Geldenhuys W, Journal Article or Conference Abstract Publication, Logan S, Metabolic syndrome, Mitochondria, Ohanyan V, Oxidative Stress, Stem, Stevanov K, Yin L Y

Histone deacetylase inhibitor vorinostat (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1beta-induced IL-6 expression in human OA chondrocytes.

AIM OF THE STUDY: High levels of IL-6 are believed to contribute to osteoarthritis (OA) pathogenesis. The expression of IL-6 is regulated post-transcriptionally by the miR-9-MCPIP-1 axis in chondrocytes. Vorinostat (SAHA) inhibits the IL-6 expression…

MicroRNA-9 promotion of interleukin-6 expression by inhibiting monocyte chemoattractant protein-induced protein 1 expression in interleukin-1beta-stimulated human chondrocytes.

OBJECTIVE: Enhanced expression of interleukin-6 (IL-6) plays an important role in the pathogenesis of osteoarthritis (OA). Monocyte chemoattractant protein-induced protein 1 (MCPIP-1) is a novel posttranscriptional regulator of IL-6 expression and is…

Tags: 2015, Academic Medical Centers, Aged, Arthritis & rheumatology (Hoboken, N.J.), Articular/drug effects/*metabolism, Cartilage, Cells, Chondrocytes – Physiology, Chondrocytes/drug effects/*metabolism, Cultured, Department of Anatomy & Neurobiology, Enzyme-Linked Immunosorbent Assay, Funding Source, Gene Knockdown Techniques, Haqqi Tariq M, Haseeb Abdul, Hip/*genetics/metabolism, Human, Humans, Immunoblotting – Utilization, Immunoprecipitation, Interleukin-1beta/pharmacology, Interleukin-6/*genetics/metabolism, Interleukins – Physiology, Makki Mohammad S, Messenger/drug effects/*metabolism, MicroRNAs/drug effects/*genetics/metabolism, Middle Aged, NEOMED College of Medicine, Ohio, Osteoarthritis, Osteoarthritis – Complications, Osteoarthritis – Physiopathology, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleases/drug effects/*genetics/metabolism, RNA, T-Tests, Transcription Factors/drug effects/*genetics/metabolism

An effective and efficient method of transfecting primary human chondrocytes in suspension.

Human chondrocytes accumulate an ECM-rich matrix by secreting matrix macromolecules during monolayer culture, which makes them difficult to transfect efficiently. Here we report a non-viral based protocol to transfect the primary human chondrocytes…

THE USE OF CULTURED EPITHELIAL AUTOGRAFTS IN THE WOUND CARE OF SEVERELY BURNED PATIENTS

Tags: 1993, Boeckman C R, burns, Cells, clinical-application, coverage, epithelial autografts, growth, human epidermal-keratinocytes, Journal Article or Conference Abstract Publication, Journal of pediatric surgery, Klein R L, McAree K G, Pediatrics, Skin, skin replacement, Surgery

Curcumin Differentially Regulates Endoplasmic Reticulum Stress through Transcriptional Corepressor SMILE (Small Heterodimer Partner-interacting Leucine Zipper Protein)-mediated Inhibition of CREBH (cAMP Responsive Element-binding Protein H)

Curcumin (diferuloylmethane), a major active component of turmeric (Curcuma longa), is a natural polyphenolic compound. Herein the effect of curcumin on endoplasmic reticulum (ER) stress responsive gene expression was investigated. We report that…

Interleukin IL-12 blocks a specific subset of the transcriptional profile responsive to UVB in epidermal keratinocytes.

Interleukin-12 (IL-12) is a proinflammatory and immunomodulatory cytokine that plays a critical role it in innate and adaptive immunity by inducing production of interferon-gamma and other cytokines. IL-12 was shown to block the ultraviolet…

Tags: *Keratinocytes/metabolism/radiation effects, *Ultraviolet Rays, 2006, Blumenberg Miroslav, Cell Culture Techniques, Cells, Cultured, Epidermal Cells, Gene Expression Regulation/drug effects, Genetic/*radiation effects, Humans, Interleukin-12/*genetics/*metabolism/pharmacology, Molecular immunology, Molenda Matthew, Mukkamala Lakshmi, Transcription

Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

Background: VEGF-regulated genes in the cervices of pregnant and non-pregnant rodents (rats and mice) were delineated by DNA microarray and Real Time PCR, after locally altering levels of or action of VEGF using VEGF agents, namely siRNA, VEGF…

Thrombospondin-4 mediates TGF-beta-induced angiogenesis.

TGF-beta is a multifunctional cytokine affecting many cell types and implicated in tissue remodeling processes. Due to its many functions and cell-specific effects, the consequences of TGF-beta signaling are process-and stage-dependent, and it is not…

Type VI collagen induces cardiac myofibroblast differentiation: implications for postinfarction remodeling.

Cardiac fibroblast (CF) proliferation and differentiation into hypersecretory myofibroblasts can lead to excessive extracellular matrix (ECM) production and cardiac fibrosis. In turn, the ECM produced can potentially activate CFs via distinct…

The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor.

BACKGROUND: Neuroinflammation is one of the hallmarks of neurodegenerative diseases, such as Parkinson's disease (PD). Activation of glial cells, including microglia and astrocytes, is a characteristic of the inflammatory response. Glycoprotein…

Cholinergic projections from the pedunculopontine tegmental nucleus contact excitatory and inhibitory neurons in the inferior colliculus

The inferior colliculus processes nearly all ascending auditory information. Most collicular cells respond to sound, and for a majority of these cells, the responses can be modulated by acetylcholine (ACh). The cholinergic effects are varied and, for…

The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.

The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or…

LPS-TLR4 Pathway Mediates Ductular Cell Expansion in Alcoholic Hepatitis.

Alcoholic hepatitis (AH) is the most severe form of alcoholic liver disease for which there are no effective therapies. Patients with AH show impaired hepatocyte proliferation, expansion of inefficient ductular cells and high lipopolysaccharide (LPS)…

Tags: 2016, Affo Silvia, Alcoholic/*immunology, Altamirano Jose, Animals, Arroyo Vicente, Bataller Ramon, Caballeria Juan, Cell Proliferation, Cells, Chen Jiegen, Collagen/genetics/metabolism, Common/*pathology, Crews Fulton, Cultured, Department of Pharmaceutical Sciences, Dumitru Raluca, Female, Gene Expression Profiling, Gene Expression Regulation, Gines Pere, Hepatic Duct, Hepatitis, Hepatocytes/*metabolism/pathology, Humans, Inbred BALB C, Keratins, Knockout, Lipopolysaccharides/metabolism, Lozano Juan Jose, Male, Matsushita Hiroshi, Mice, Middle Aged, Morales-Ibanez Oriol, NEOMED College of Pharmacy, Odena Gemma, Rautou Pierre-Emmanuel, Rodrigo-Torres Daniel, Sancho-Bru Pau, Scientific reports, Seki Ekihiro, Signal Transduction, Toll-Like Receptor 4/genetics/*metabolism, Type I/genetics/*metabolism, Valla Dominique, You Min, Zou Jian

Endothelin-mediated in vivo pressor responses following TRPV1 activation.

Transient receptor potential vanilliod 1 (TRPV1) channels have recently been postulated to play a role in the vascular complications/consequences associated with diabetes despite the fact that the mechanisms through which TRPV1 regulates vascular…

Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol.

Cardiac fibroblasts (CFs) regulate myocardial remodeling by proliferating, differentiating, and secreting extracellular matrix proteins. Prolonged activation of CFs leads to cardiac fibrosis and reduced myocardial contractile function. Resveratrol…

Tags: 2005, 70-kDa/metabolism, American journal of physiology. Heart and circulatory physiology, Angiotensin II/pharmacology, Animals, Antioxidants/*pharmacology, Bomser Joshua A, Cell Differentiation/drug effects, Cell Division/drug effects, Cells, Cultured, Department of Integrative Medical Sciences, Drug Interactions, Epidermal Growth Factor/pharmacology, Fibroblasts/*cytology/*drug effects, Male, Meszaros J Gary, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3/metabolism, Myocardium/*cytology, Naugle Jennifer E, NEOMED College of Medicine, Olson Erik R, Protein-Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins c-akt, Proto-Oncogene Proteins/metabolism, Rats, Resveratrol, Ribosomal Protein S6 Kinases, Signal Transduction/drug effects, Sprague-Dawley, Stilbenes/*pharmacology, Vasoconstrictor Agents/pharmacology, Zhang Xiaojin

Angiotensin II-induced extracellular signal-regulated kinase 1/2 activation is mediated by protein kinase Cdelta and intracellular calcium in adult rat cardiac fibroblasts.

Angiotensin II (Ang II)-induced proliferation of cardiac fibroblasts is a major contributing factor to the pathogenesis of cardiac fibrosis. Ang II activates extracellular signal-regulated kinase (ERK) 1/2 to induce cardiac fibroblast proliferation,…

Extracellular ATP and Toll-Like Receptor 2 Agonists Trigger in Human Monocytes an Activation Program That Favors T Helper 17

Strategically-paired Toll-like receptor (TLR) ligands induce a unique dendritic cell (DC) phenotype that polarizes Th1 responses. We therefore investigated pairing single TLR ligands with a non TLR-mediated danger signal to cooperatively induce…

Effect of Transendocardial Delivery of Autologous Bone Marrow Mononuclear Cells on Functional Capacity, Left Ventricular Function, and Perfusion in Chronic Heart Failure The FOCUS-CCTRN Trial

Context Previous studies using autologous bone marrow mononuclear cells (BMCs) in patients with ischemic cardiomyopathy have demonstrated safety and suggested efficacy. Objective To determine if administration of BMCs through transendocardial…

Tags: 2012, acute myocardial-infarction, Aguilar D, Anderson R D, Anwaruddin S, Baraniuk S, Bettencourt J, Bowman S, Cardiovasc Cell Therapy Res Networ, Cells, Chen J, Cogle C R, coronary-artery-disease, Ebert R F, Ellis S G, f-18, fluorodeoxyglucose, Francescon J, Gee A P, Geither C, General & Internal Medicine, Gordon D J, Handberg E, Hatzopoulos A K, Henry T D, intramyocardial injection, Jama-Journal of the American Medical Association, Journal Article, Kronenberg M W, Kwak M, Lai D J, Loghin C, Martin A D, metabolic-activity, Moye L A, Olson R E, Penn M S, Pepine C J, Perin E C, Piller L B, progenitor, randomized controlled-trial, research network cctrn, Richman S, Sayre S L, Silva G V, Simari R D, Simpson L M, Skarlatos S I, Smith D, stem-cells, Taylor D A, Thomas J D, transcoronary transplantation, Traverse J H, Vojvodic R W, Westbrook L, Willerson J T, Zhao D X M, Zierold C

Immune response to nonspecific and altered tissue antigens in soft tissue allografts.

Soft tissue allografts have many uses in orthopaedic surgery, including knee ligament reconstruction, hand tendon surgery, shoulder instability, and rotator cuff reconstruction. The predictable biologic incorporation of soft tissue allografts without…

Neutrophil elastase downmodulates native G-CSFR expression and granulocyte-macrophage colony formation

Background: The granulocyte colony-stimulating factor receptor (G-CSFR) plays a critical role in maintaining homeostatic levels of circulating neutrophils (PMN). The mechanisms modulating G-CSFR surface expression to prevent chronic neutrophilia are…

Stem Cell Therapy for Heart Disease

Coronary artery disease is the leading cause of death in Americans. After myocardial infarction, significant ventricular damage persists despite timely reperfusion and pharmacological management. Treatment is limited, as current modalities do not…

Mitochondrial oxidative stress corrupts coronary collateral growth by activating adenosine monophosphate activated kinase-alpha signaling.

OBJECTIVE: Our goal was to determine the mechanism by which mitochondrial oxidative stress impairs collateral growth in the heart. APPROACH AND RESULTS: Rats were treated with rotenone (mitochondrial complex I inhibitor that increases reactive oxygen…

Tags: 2013, AMP-Activated Protein Kinases/*metabolism, Animal, Animals, Arteriosclerosis, thrombosis, and vascular biology, Body Weight/physiology, Cells, Chen Chwen-Lih, Chen Yeong-Renn, Chilian William M, Collateral Circulation, Coronary Circulation, Coronary Vessels/cytology/*enzymology, Cultured, Department of Integrative Medical Sciences, Disease Models, Dyck Jason R B, Endothelial Cells/cytology/*enzymology, Enrick Molly, Hardwick James P, Humans, Inbred WKY, Ischemia/metabolism/pathology, Kolz Christopher, Mitochondria, Mitochondria/drug effects/*metabolism, Myocardium/enzymology/pathology, NEOMED College of Medicine, Oxidative Stress/*physiology, Pung Yuh Fen, Rats, reactive oxygen species, Rotenone/pharmacology, Sam Wai Johnn, Signal Transduction/*physiology, Stevanov Kelly, Thakker Prashanth, TOR Serine-Threonine Kinases/metabolism, Uncoupling Agents/pharmacology, Yin Liya

Mechanisms of Chinese Medicine Xinmailong's protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes.

Patients with heart failure (HF) have high mortality and mobility. Xinmailong (XML) injection, a Chinese Medicine, is clinically effective in treating HF. However, the mechanism of XML's effectiveness on HF was unclear, and thus, was the target of…

Optimal reactive oxygen species concentration and p38 MAP kinase are required for coronary collateral growth.

Reactive oxygen species (ROS) are implicated in coronary collateral growth (CCG). We evaluated the requirement for ROS in human coronary artery endothelial cell (HCAEC) tube formation, CCG in vivo, and signaling (p38 MAP kinase) by which ROS may…

Tags: *Collateral Circulation/drug effects, *Coronary Circulation/drug effects, *MAP Kinase Signaling System/drug effects, 2007, Acetophenones/pharmacology, American journal of physiology. Heart and circulatory physiology, Animal, Animals, Blood Flow Velocity, Cells, Chilian William M, Coronary Vessels/surgery, Cultured, Department of Integrative Medical Sciences, Disease Models, Ditiocarb/pharmacology, Endothelial Cells/drug effects/enzymology/*metabolism, Enzyme Inhibitors/pharmacology, Humans, Imidazoles/pharmacology, Inbred WKY, Kolz Christopher, Ligation, Male, Myocardial Reperfusion Injury/enzymology/metabolism/*physiopathology, NADPH Oxidases/antagonists & inhibitors/metabolism, NEOMED College of Medicine, Neovascularization, Onium Compounds/pharmacology, Oxygenases/antagonists & inhibitors/metabolism, p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism, Physiologic, Potter Barry, Pyridines/pharmacology, Rats, Reactive Oxygen Species/*metabolism, Reed Ryan, Rocic Petra, Superoxide Dismutase/antagonists & inhibitors/metabolism, Vascular Endothelial Growth Factor A/metabolism

Browse Items (117 total)